Genes differentially expressed in lung cancer

在过去的一年里，我们使用原位杂交的方法来评估SAGE和cDNA阵列分析所选择的候选基因的相对表达。我们使用新鲜的肺癌和周围正常组织的冰冻切片，并将该组织与生物素标记的候选基因探针杂交。然后使用基于酪胺的方法将杂交信号可视化。到目前为止，我们已经调查了近50个新鲜的冷冻肺肿瘤样本，其中21个适合进行原位杂交。利用这些组织，我们筛选了五个基因在肺癌中的差异表达，并确定了一个潜在的肿瘤标记物，该标记物在90%被检查的肿瘤中特异表达，但在正常肺中不存在。我们目前正在测定这一候选标记物在大量石蜡包埋的肺组织中的相对表达，以确定该基因在肺癌中的表达的临床意义。我们还在筛选其他在肺癌中差异表达的基因。

Over the past year, we have used in situ hybridization method to assess the relative expression of candidate genes selected by SAGE and cDNA array analysis. We used fresh frozen tissue sections from lung cancers with surrounding normal and hybridize the tissue with biotin labeled probes of candidate genes. The hybridization signal is then visuallized using a tyramide based method. We have thus far surveyed nearly 50 fresh frozen lung tumor samples and 21 of those were suitable for in situ hybridization. Using these tissues, we have screened five genes for their differential expression in lung cancers and identified a potential tumor marker that is expecifically expressed in 90% of the tumors examed but not present in the normal lung. We are currently determining the relative expression of this candidate marker in a large panel of paraffin embedded lung tissues to determine the clinical significance of this gene's expression in lung cancer. We are also screening additional genes that are differentially expressed in lung cancer.