High Throughput Proteome Analysis of Schistosoma mansoni

描述（由申请人提供）：蛋白质组学分析对于更好地理解寄生虫生物学的基本方面、分配从生物体基因组序列中鉴定的许多基因的功能以及提供有助于基因组序列注释的信息至关重要。这是一个建议，使用高通量蛋白质组学的方法来表征蛋白质的补充，是（1）分泌的曼氏血吸虫卵（2）存在于成人的表面上的S。（3）转化曼氏血吸虫分泌的蛋白蠕虫;曼氏尾蚴。我们将在蛋白质组学分析之后详细研究卵分泌蛋白。这项研究将使我们能够测试这一假设，即鸡蛋释放的蛋白质在保护鸡蛋免受免疫介导的破坏方面发挥重要作用，在组织通道中很重要，并且是肉芽肿形成的主要免疫刺激剂。分泌蛋白和表面蛋白的表征将增加我们对染色体的基本生物学的理解。我们将鉴定S. mansoni促进入侵，存活和穿过宿主组织，并阐明肉芽肿形成的免疫学基础以及寄生虫如何感知它们的环境并与它们的配偶交流。这些结果可能被证明是有用的战略，以改善病理反应，血吸虫病和新的诊断，药物和疫苗的发展。本申请中描述的高通量鸟枪蛋白质组学项目将使用多维蛋白质鉴定技术（MudPIT）分析蛋白质混合物。该项目的完成将提供关于S. mansoni的，也验证了使用MudPIT在未来的蛋白质组学研究，以确定一个全面的看法，S。mansoni蛋白质组

DESCRIPTION (provided by the applicant): Proteomic analyses will be essential to better understand fundamental aspects of parasite biology, to assign the function of many genes identified from the genome sequence of an organism, and will provide information to aid in the annotation of the genome sequence. This is a proposal to use a high throughput proteomics approach to characterize protein complements that are (1) secreted by the eggs of Schistosoma mansoni (2) present on the surface of adult S. mansoni worms, and (3) secreted by transforming S. mansoni cercariae. We will follow the proteomic analysis with a detailed investigation of egg-secreted proteins. This study will allow us to test the hypothesis that proteins released by eggs play significant roles in protecting the egg from immune-mediated destruction, are important in tissue passage, and are major immuno-stimulants of granuloma formation. The characterization of secreted and surface proteins will increase our understanding of the basic biology of schistosomes. We will identify proteins used by S. mansoni to promote invasion, survival, and passage across host tissues and shed light on the immunological basis of granuloma formation and how schistosomes sense their environment and communicate with their mate. These results may prove to be useful in strategies to ameliorate pathologic responses in schistosomiasis and in the development of novel diagnostics, drugs and vaccines. The high throughput shotgun proteomics project described in this application will analyze protein mixtures using multidimensional protein identification technology (MudPIT). Completion of this project will provide information on significant protein complements of S. mansoni and also validate the use of MudPIT in future proteomic studies to define a comprehensive view of the S. mansoni proteomes.