Micromass QTof Hybrid Mass Spectrometer

描述(由申请人提供)：请求为NCRR提供微质量混合四极飞行时间(Q ToF)质谱仪的共享仪器赠款。该仪器将被安置在伯克利校区的一个新的研究单位--整合基因组学中心(CIG)。该仪器将用于一维或二维凝胶电泳后的蛋白质鉴定，使用蛋白质水解肽的MS-MS测序。该仪器支持的项目包括人类和四膜虫端粒酶、果蝇、乔纳和阿尔特米亚转录因子、果蝇P元素转座酶和DNA修复蛋白、果蝇RNA结合/RNA剪接因子以及非洲爪哇胚胎转录和信号转导因子。该仪器还将用于分析转录因子、DNA重组酶、端粒酶和RNA剪接因子的蛋白质磷酸化，使用纳米LC MS-MS和前驱体离子扫描以正负模式进行分析。加州大学伯克利分校没有这种类型的仪器，它使用纳米电喷雾电离和混合四极飞行时间质量分析仪。

DESCRIPTION (provided by applicant): A request is made for a shared instrumentation grant to NCRR for a Micromass hybrid quadrupole time of flight (Q ToF) mass spectrometer. The instrument will be housed in the Center for Integrated Genomics (CIG), a new research unit on the Berkeley campus. The instrument will be used for protein identification following one- or two-dimensional gel electrophoresis, using MS-MS sequencing of proteolytic peptides. Projects supported by this instrument will involve human and Tetrahymena telomerase, Drosophila, Ciona and Artmia transcription factors, Drosophila P element transposase and DNA repair proteins, Drosophila RNA binding / RNA splicing factors and Xenopus embryonic transcription and signal transduction factors. The instrument will also be used to analyze protein phosphorylation of transcription factors, DNA recombination enzymes, telomerase and RNA splicing factors using nano-LC MS-MS and precursor ion scanning in the positive or negative mode. An instrument of this type, using nano-elctrospray ionization in conjunction with a hybrid quadrupole-time-of-flight mass analyzer is not available on the U.C., Berkeley campus.