BD FACS Vantage II Cell Sorter

描述(由申请者提供)：Sidney Kimmel癌症中心是一个非营利性研究机构，成立于1991年，由USPH赠款资助的癌症基因组学、血管生成和血管生物学、造血和免疫学以及分子和细胞生物学的研究项目。该中心已迅速发展到我们目前的21名首席调查人员和130名支持人员，每年为NIH提供1200万美元的支持。目前，西德尼·基梅尔癌症中心的员工占地90,000平方米。英国《金融时报》毗邻38,000平方米的建筑。英国《金融时报》在建大楼，2003年7月入驻。一个流式细胞仪核心设施正在扩建中。目前，该设施包括一台Becton Dickinson FACS Calibur仪器，配备有四个荧光参数检测设备，以及位于整个中心的五个计算机工作站，这些工作站安装有Cell Quest数据分析软件，并与具有24 GB存储容量的NT Windows服务器联网。目前，每月都有针对新工作人员的培训班，关于流式细胞仪各种用途的系列研讨会正在开发中。收购B.D.FACS Vantage SE II细胞仪将为该中心的用户以及圣地亚哥地区更大的研究社区提供最先进的荧光激活细胞分选能力。西德尼·基梅尔癌症中心已经确定了8名主要用户和6名次要用户，他们的研究计划需要或将因获得这项技术而大大增强。所有的主要用户和大多数次要用户都有使用流式细胞仪的经验，许多人经常使用附近研究机构的细胞分选设施。大多数用户需要收集大量具有特定表型的纯化细胞用于各种体外和体内分析，其他用户需要筛选和选择成功转基因的细胞系、细菌，或从大样本中纯化非常稀有的细胞用于基因表达分析。FACS Vantage SE II仪器具有多参数(高达8个荧光参数和2个光散射参数)检测能力、允许同时收集四个子集的4路分选、自动分选到多孔托盘和每秒25,000个事件的分选速度，同时保持出色的生存能力、功能和纯度，是这些需求的理想选择。

DESCRIPTION (provided by applicant): The Sidney Kimmel Cancer Center is a nonprofit research institute founded in 1991 with USPH grant-supported research programs in cancer genomics, angiogenesis and vascular biology, hematopoiesis and immunology and molecular and cell biology. The Center has grown rapidly to our current roster of 21 principal investigators and 130 support staff with $12 million/year in NIH support. Presently employees of the Sidney Kimmel Cancer Center occupy a 90,000 sq. ft. building with an adjacent 38,000 sq. ft. building under construction, to be occupied in July 2003. A flow cytometry core facility is under expansion. Presently the facility consists of a Becton Dickinson FACS Calibur instrument equipped for four fluorescence parameter detection and five computer workstations located throughout the Center having Cell Quest data analysis software and networked to a NT Windows server having a 24 gigabyte storage capacity. Currently there are monthly training classes for new staff and a seminar series on the various uses of flow cytometry is under development. The acquisition of a B.D. FACS Vantage SE II cytometer would provide state-of-the art fluorescence-activated cell sorting capability, both to users at the Center but also to the larger research community in the San Diego area. Eight major and six minor users have been identified at the Sidney Kimmel Cancer Center whose research programs either require or would be significantly enhanced by access to this technology. All of the major users and most of the minor users have experience with flow cytometry and many routinely utilize cell sorting facilities at neighboring research institutes. The majority of the users have need to collect large numbers of purified cells with a defined phenotype for use in a variety of in vitro and in vivo assays, others require the screening and selection of successfully transfected cell lines, bacteria, or the purification of very rare cells from large samples for gene expression analyses. The FACS Vantage SE II instrument would be ideally suited to these needs with its capacity for multi-parameter (up to 8 fluorescence and 2 light scatter parameters) detection, 4-way sorting allowing the simultaneous collection of four subsets, automated sorting into multi-well trays and sorting speeds of 25,000 events/second with retention of excellent viability, function and purity.