CORE--Cellular and Molecular Physiology Core

该核心设施旨在为希望从事以下工作的肝脏中心研究人员提供技术专业知识、设备和人员：a)分离细胞制剂，包括：肝细胞， 主要来自大鼠和小鼠的胆管细胞、内皮细胞、星状细胞和肝淋巴细胞b)短期和长期培养的细胞培养设施和细胞系c)利用实时定量PCR机和荧光成像设备进行研究的分离肝脏灌流制剂，或e)使用IS RNA和腺病毒转染技术改变这些细胞和组织中的基因表达。通过将这些程序集中在一个核心设施中，调查人员可以确保高度的质量控制，并且制剂通常可以由多名调查人员同时使用。e)位于沙漠山生物实验室的中心？S卫星设施也建立了服务a-c，用于使用海洋物种的替代模型，主要是小型溜冰鱼。

This Core facility is organized to provide technical expertise, equipment and personnel to Liver Center Investigators who wish to work with: a) Isolated cell preparations including: hepatocytes, cholangiocytes, endothelial cells, stellate cells and hepatic lymphocytes, primarily from rat and mouse b) Cell culture facilities for short and long term cultures and cell lines c) Isolated liver perfusion preparations for studies utilizing the whole organ d gene expression using Quantitative real time PCR machine and Phosphor-imaging equipment or e) Alter expression of genes in these cells and tissues using is RNA and adenovirus transfection technologies. By centralizing these procedures in a Core facility, investigators are assured of a high degree of quality control and preparations can often be used simultaneously by more then one investigator, e) Services a-c are also established at the Center?s satellite facility at the Mount Desert Island Biological Laboratory for use of alternative models in marine species, primarily the small skate, Raja erinacea.