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Structural Biology of sulfotransferase and glycosyltrans

Structural Biology of sulfotransferase and glycosyltrans
磺基转移酶和糖基反式的结构生物学
批准号：
6838567
负责人：
MASAHIKO NEGISHI
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/6838567
关键词：
X ray crystallography environmental toxicology enzyme activity enzyme structure enzyme substrate complex estrogens genetic susceptibility glucuronosyltransferase glycosyltransferase hydroxysteroids protein protein interaction site directed mutagenesis structural biology sulfotransferase

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中文摘要
翻译
胞质STs是用于解毒和分泌的硫酸盐异种化学物质，也是用于灭活和/或储存的硫酸盐生物信号分子，如类固醇激素和生物胺。某些硫酸盐制品会致癌。高尔基膜STs对多糖的不同位点具有高特异性。各种糖基转移酶合成多糖并为STs提供底物。我们的研究目标是阐明这些酶的结构和功能。为此，我们利用细菌表达系统产生了大量的重组蛋白，并对其进行了结晶化，求解了其三维结构。
英文摘要
Cytosolic STs sulfate xenochemicals for detoxification and secretion and also sulfate biological signal molecules such as steroid hormones and bioamines for inactivation and/or storage. Certain sulfated products become carcinogenic. Golgi-membrane STs display high specificity to sulfate distinct sites of polysaccharides. Various glycosyltransferases synthesize polysaccharides and provide STs with substrates. Our research objectives are to eluciadte structure and function of these enzymes. For this, we have used bacterial expression system to produce a large amount of recombinant proteins, have crystallized them, and have solved their 3-dimensional structures.
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