Study To Assess The Utility Of Oral Washes To Diagnose P

这项研究是一项为期15年的项目的一部分，该项目旨在开发微创方法来诊断肺囊虫性肺炎并预测治疗反应。收集了免疫抑制性疾病和呼吸综合征患者的口腔洗液、诱导痰和支气管肺泡灌洗。在这项试验中，研究结果已经从关注组织转移到关注呼吸道分泌物，特别是可以非侵入性获得的分泌物。前瞻性地收集了旧金山总医院感染艾滋病毒和可能感染肺囊虫性肺炎的患者的口腔洗液。定量PCR在鉴别肺囊虫肺炎患者方面具有高灵敏度和高特异性，特别是在使用50拷贝/uL的截止值时。本研究完成。进一步的研究使用改进的方法来溶解临床标本正在进行中。

This study is part of a 15-year project to develop less invasive methods to diagnose pneumocystis pneumonia and to predict responses to therapy. Oral washes, induced sputum, and bronchalveolar lavage have been collected from patients with immunosuppressive diseases and respiratory syndromes. During this trial resulting studies have moved the field from a focus on tissue to a focus on respiratory secretions, especially secretions that can be obtained non-invasively. Oral washes have been collected prospectively from patients at San Francisco General Hospital who have HIV infection and possible pneumocystis pneumonia. Quantitative PCR had a high sensitivity and high specificity for identifying patients with pneumocystis penumonia, especially if a cut-off of 50 copies/uL were used. This sutdy is completed. Further studies using improved methods to solubulize the clinical specimen are underway.