Regulation of Prostaglandins in Periovulatory Follicles

描述(申请人提供)：黄体生成素/卵泡刺激素激波启动级联反应 最终导致卵泡壁显著破裂的事件 卵母细胞-卵丘复合体的顶端和随后的释放。尽管它的 对生殖和生育至关重要，尽管几十年来 研究表明，我们对生物多样性的调控知识还存在许多空白 排卵过程。许多调节器和途径被假设为 与哺乳动物排卵有关，但之前的研究结果 强调了黄体酮和前列腺素(PG)的重要作用。黄牛 为研究地球表面的复杂事件提供了理想的动物模型。 排卵期。我们已经验证了一个诱导排卵的模型。 牛的卵泡发育，我们现在建议用它来测试 关于排卵的这两个关键调节因子及其受体的假说。在……里面 特异性目标1和2，合成的时机，细胞位置(S)，生物合成 围排卵期PG合成增加的途径和激素调节 将会被检查。需要检验的假设是卵泡膜和颗粒膜都 细胞对排卵前促黄体生成素/卵泡刺激素激增的反应是PG增加 产生，由浪涌诱导的孕酮升高，而孕酮反过来 通过从PGE2还原来诱导PGF2a的合成。在具体目标3中，我们将 检验孕酮对前列腺素影响的假设 排卵周卵泡的产生至少部分是由 通过黄体酮诱导催产素的增加。虽然控球是必不可少的 对于排卵，几乎对PG受体的表达一无所知。 在排卵期。在具体目标4中，时间表达 牛排卵周围卵泡中PGF2α受体的mRNA将 下定决心。受体信使核糖核酸协同表达的假说 随着PG合成的增加，和，是定位于膜细胞的 将对排卵周卵泡进行检测。《特定目标5》旨在测试 LWFSH峰诱导孕酮受体(PR)的假说 排卵周围卵泡的卵泡细胞及其受体介导 黄体酮对PG产生的影响。这些实验加在一起将 提供关于这些的监管以及它们之间关系的新知识 排卵的两种重要介质及其受体。

DESCRIPTION (provided by applicant): The LH/FSH surge sets in motion a cascade of events that culminates in the remarkable rupture of the follicular wall at the apex and the subsequent release of the oocyte-cumulus complex. Despite its critical importance to reproduction and fertility, and despite decades of research, there are many gaps in our knowledge of the regulation of the ovulatory process. Many regulators and pathways have been hypothesized to be involved in mammalian ovulation, but results of previous studies have highlighted essential roles for progesterone and prostaglandins (PGs). Cattle provide an ideal animal model for studying the complex events of the periovulatory period. We have validated a model for inducing periovulatory follicular development in cattle and we now propose to use it to test hypotheses about these two key regulators of ovulation and their receptors. In Specific Aims 1 and 2, the timing, cellular site(s) of synthesis, biosynthetic pathway, and hormonal regulation of the periovulatory increase in PG synthesis will be examined. The hypothesis to be tested is that both theca and granulosa cells respond to the preovulatory LH/FSH surge with an increase in PG production, mediated by the surge-induced rise in progesterone, which in turn induces PGF2a synthesis, via reduction from PGE2. In Specific Aim 3 we will test the hypothesis that the effects of progesterone on prostaglandin production by the periovulatory follicle are at least partially mediated through progesterone-induced increases in oxytocin. Although PGs are essential for ovulation, virtually nothing is known about the expression of PG receptors during the periovulatory period. In Specific Aim 4, the temporal expression of mRNA for PGF2alpha receptor in bovine periovulatory follicles will be determined. The hypothesis that mRNA for the receptor is expressed coordinately with the rise in PG synthesis and, is localized to the theca cells of the periovulatory follicle will be tested. Specific Aim 5 is designed to test the hypothesis that the LWFSH surge induces progesterone receptor (PR) in follicular cells of the periovulatory follicle and that these receptors mediate progesterone's effects on PG production. Together these experiments will provide new knowledge about the regulation of, and relationship between, these two essential mediators of ovulation and their receptors.