High Throughput Proteome Analysis of Schistosoma mansoni

描述（由申请人提供）：蛋白质组学分析对于更好地理解寄生虫生物学的基本方面，分配从生物体基因组序列中鉴定的许多基因的功能，并将为基因组序列的注释提供信息是必不可少的。这是一项使用高通量蛋白质组学方法来表征蛋白质补体的建议，这些蛋白质补体是(1)由曼氏血吸虫的卵分泌的(2)存在于成年曼氏血吸虫的表面，以及(3)由转化曼氏血吸虫尾蚴分泌的。我们将继续进行蛋白质组学分析，详细研究鸡蛋分泌蛋白。这项研究将使我们能够验证鸡蛋释放的蛋白质在保护鸡蛋免受免疫介导的破坏方面发挥重要作用，在组织传递中发挥重要作用，并且是肉芽肿形成的主要免疫刺激物。分泌蛋白和表面蛋白的表征将增加我们对血吸虫基本生物学的理解。我们将鉴定曼氏血吸虫用于促进入侵、存活和穿越宿主组织的蛋白质，并阐明肉芽肿形成的免疫学基础，以及血吸虫如何感知环境并与配偶沟通。这些结果可能对改善血吸虫病病理反应的策略以及开发新的诊断方法、药物和疫苗有用。本应用程序中描述的高通量霰弹枪蛋白质组学项目将使用多维蛋白质鉴定技术（MudPIT）分析蛋白质混合物。该项目的完成将提供mansoni重要蛋白质补体的信息，并验证MudPIT在未来蛋白质组学研究中的使用，以定义mansoni蛋白质组学的综合视图。

DESCRIPTION (provided by the applicant): Proteomic analyses will be essential to better understand fundamental aspects of parasite biology, to assign the function of many genes identified from the genome sequence of an organism, and will provide information to aid in the annotation of the genome sequence. This is a proposal to use a high throughput proteomics approach to characterize protein complements that are (1) secreted by the eggs of Schistosoma mansoni (2) present on the surface of adult S. mansoni worms, and (3) secreted by transforming S. mansoni cercariae. We will follow the proteomic analysis with a detailed investigation of egg-secreted proteins. This study will allow us to test the hypothesis that proteins released by eggs play significant roles in protecting the egg from immune-mediated destruction, are important in tissue passage, and are major immuno-stimulants of granuloma formation. The characterization of secreted and surface proteins will increase our understanding of the basic biology of schistosomes. We will identify proteins used by S. mansoni to promote invasion, survival, and passage across host tissues and shed light on the immunological basis of granuloma formation and how schistosomes sense their environment and communicate with their mate. These results may prove to be useful in strategies to ameliorate pathologic responses in schistosomiasis and in the development of novel diagnostics, drugs and vaccines. The high throughput shotgun proteomics project described in this application will analyze protein mixtures using multidimensional protein identification technology (MudPIT). Completion of this project will provide information on significant protein complements of S. mansoni and also validate the use of MudPIT in future proteomic studies to define a comprehensive view of the S. mansoni proteomes.