Ocular fluorophotometer for use with small eyes

描述（由申请人提供）：该SBIR项目的目的是开发一种用于测量小鼠眼睛的眼部荧光光度计。 事实证明，眼部荧光光度法是眼科研究中一种有用且通用的技术。许多眼部疾病的小动物模型，特别是基因工程小鼠，提供了一个独特的机会。 我们建议发展一个系统来执行小鼠的眼睛荧光光度计。在整个项目过程中（I期和II期），我们打算开发和测试用于测量血视网膜屏障渗透性、血房水屏障渗透性、房水周转、角膜内皮渗透性和角膜上皮渗透性的仪器和方法。 在第一阶段，我们将把我们的努力限制在测量水循环，但正如我们将解释的那样，这将为其他应用奠定基础，并充分回答这些应用的可行性问题。 在第二阶段，我们将处理其他应用程序，并进一步完善第一阶段设计的方法。在第一阶段开发的一个领域将在第二阶段保持重点，这将是动物约束系统。我们的最终目标是开发一个系统，可以快速筛查许多受试者。

DESCRIPTION (provided by applicant): The purpose of this SBIR project is to develop an ocular fluorophotometer for measurement in the mouse eye. Ocular fluorophotometry has proven to be a useful and versatile technique in eye research. Small animal models of numerous ocular diseases, particularly in genetically engineered mice, present a unique opportunity. We propose to develop a system for performing ocular fluorophotometer on the mouse. Over the course of the entire project (both Phase I and Phase II), we intend to develop and test instrumentation and methods for measuring blood retinal barrier permeability, blood aqueous barrier permeability, aqueous turnover, cornea endothelial permeability and cornea epithelial permeability. During Phase I, we will limit our efforts to the measurement of aqueous turnover, but as we will explain, this will lay the foundation for the other applications and adequately answer the question of feasibility for those applications. During Phase II, we will address the other applications as well as further refine methods designed during Phase I. One area developed in Phase I that will remain a focus during Phase II will be the animal restraint system. It is our ultimate goal to develop a system that will allow rapid screening of many subjects.