CORE--BIOMATERIALS

PKD生物材料研究核心的总体目标是建立和维护人类和动物生物材料库。堪萨斯PKD中心的调查人员该中心将为高质量研究材料的开发提供资源和专业知识，包括上皮细胞、组织学标本、DNA、RNA和细胞蛋白的原代培养，这些原代培养来自ADPKD、ARPKD和正常人类肾脏组织。该核心还将建立和维持来自具有独特PKD基因突变的小鼠的永生化细胞系。核心的优势在于，它将提供一个中央实验室，配备专门的研究人员，经验丰富：1)采购人体组织，并接受过正确处理人体生物样品的培训；2)无菌收集囊性和非囊性组织和囊液的方法；

The overall goal of the PKD Biomaterials Research Core is to establish and maintain a repository for human and animal biological materials for.investigators within the Kansas PKD Center. The core will provide resources and expertise for the development of high quality research materials, including primary cultures of epithelial cells, histological specimens, DNA, RNA and cell proteins from ADPKD, ARPKD and normal human kidney tissues. The core will also establish and maintain immortalized cell lines from mice with unique PKD gene mutations. The strength of the core is that it will provide a central laboratory with dedicated research staff experienced in 1) the procurement the human tissues and trained on the proper handling of the human biological samples, 2) methods of aseptic collection of cystic and non-cystic tissue and cyst fluid, 3) histological preparation 4) protein, RNA and DNA extraction, 5) animal husbandry, timed breeding and animal genotyping, and 6) preparation of cell lines from specific cell types for PKD mice. The specific aims of the Core are: Aim 1: Establish and maintain a human PKD biomaterial repository. Aim 2: Generate and maintain conditionally immortalized renal epithelial cell cultures from animals with unique gene defects that cause PKD. Aim 3: Provide technical support and training for in vitro methods for the investigation of the PKD cellular phenotype. The Core will provide unique PKD biomaterials to members of the Kansas PKD Center and to collaborators at the Kansas University Medical Center and other universities.