CORE--OK COBRE: NUCLEIC ACID ANALYSIS

核酸分析核心的能力为初级研究人员（和较大的机构）提供了一个单一的切入点，为核酸实验操作的技术方面提供全面的建议（指导），获得训练有素的技术人员和非常复杂和昂贵的设备。在某些情况下，将获得建议和教育。对于大多数核心将提供检测开发，数据收集或核苷酸合成。该核心集中了高效可靠地生成高质量核酸分析数据所需的设备和经验。通过该核心提供的服务包括DNA测序、单核苷酸多态性（SNP）基因分型、小鼠和人的表达阵列以及SNP阵列。与每个初级研究者的互动将根据需要进行调整，以满足每个人的个人培训需求。例如，作为一名实验学家，纳特博士的经验不如格思里奇博士。因此，与Guthridge博士的互动将集中在效率和生产力上。与此相反，与纳特博士的互动将包括对纳特博士（及其工作人员）的培训， 在他的实验室里开发的。这意味着我们将鼓励青年积极参与 研究者参与核心的操作，我们将阻止初级研究者对核心的被动依赖。核心服务用于高效、经济地提供数据和服务。在某些情况下，将生成大量高吞吐量数据，例如为Sestak博士领导的项目生成的数百万个数据点。核酸分析核心将为COBRE、其初级研究员、OMRF和更大的当地俄克拉荷马州卫生中心研究社区提供许多最先进的服务。

The capabilities assembled in the Nucleic Acid Analysis Core provide the Junior Investigators (and larger institution) a single point of entry, comprehensive advice (mentoring) for the technical aspects of experiments manipulating of nucleic acids, access to highly-trained technical personnel and very sophisticated and expensive equipment. In some situations advice and education will be obtained. For most the Core will offer assay development, data collection, or nucleotide synthesis. This Core centralizes the equipment and experience needed to efficiently and reliably produce high quality nucleic acid analysis data. The services available through this Core include DNA sequencing, single nucleotide polymorphism (SNP) genotyping, expression arrays in mouse and man, and SNP arrays. The interaction with each of the Junior Investigators will be tailored so that the individual training needs of each are met. For example, Dr. Nath is less experienced as an experimentalist than is Dr. Guthridge. Consequently, the interaction with Dr. Guthridge will be focused upon efficiency and productivity. In contrast, the interaction with Dr. Nath will include training for Dr. Nath (and for his staff) so that the technological expertise will be developed in his laboratory. This means that we will encourage active involvement of the Junior Investigators in the operation of the Cores and we will discourage passive dependence upon the Cores by Junior Investigators. The core serves to provide data and services efficiently and economically. In some situations massive high throughput data will be generated, such as the millions of data points generated for the project being led by Dr. Sestak. The Nucleic Acid Analysis Core will offer many state-of-the-art services to the COBRE, its Junior Investigators, the OMRF, and the larger local Oklahoma Health Center research community.