Fluorescence microscopy image acquisition/analysis sys

描述(由申请人提供)：对整个细胞以及分离的细胞骨架元素和细胞器进行最先进的成像，对于实现NIH授予参与这项共享仪器拨款计划的六名加州大学伯克利分校细胞生物学家的具体目标至关重要。使用最新技术获取和分析荧光图像已成为这些研究项目继续取得成功的关键。这项提议的总体目标是确保获得资金，以获得最先进的奥林巴斯成像系统，其功能目前申请者无法获得，或者需求如此之高，以至于现有共享系统的时间竞争严重阻碍了进展。该成像系统将提供的能力包括全内反射(TIRF)显微镜、用于实时分析动态细胞过程的高速多色图像采集和去卷积显微镜。申请资助的具体系统，以及该系统的能力如何与每个研究人员的NIH资助的研究计划的目标相关联，在本提案的正文中进行了详细描述。

DESCRIPTION (provided by applicant): State-of-the-art imaging of whole cells and of isolated cytoskeletal elements and organelles is vital to achieving the Specific Aims of the NIH grants awarded to each of the six U.C. Berkeley cell biologists participating in this Shared Instrumentation Grant proposal. Acquisition and analysis of fluorescence images using the latest technologies has become essential to the continued success of each of these research programs. The overall aim of this proposal is to secure funds to acquire a state-of-the-art Olympus imaging system with capabilities that are currently unavailable to the applicants, or that are in such high demand that progress is being significantly hindered by competition for time on the one existing shared system. The capabilities to be provided by this imaging system include Total Internal Reflection (TIRF) microscopy, high-speed multi-color image acquisition for real time analysis of dynamic cellular processes, and deconvolution microscopy. The specific system for which funding is being requested, and how the capabilities of this system relate to the Aims of the NIH-funded research program of each investigator, are described in detail in the body of this proposal.