Rutgers University Cell and DNA Repository Renovation

描述（由申请人提供）：我们要求资金翻新和改善现有空间，为罗格斯大学细胞和DNA储存库（RUCDR）提供实验室和办公区，这是四个NIH研究所的指定核心生物样品处理和储存设施;酒精和酒精滥用;消化，糖尿病和肾脏疾病;药物滥用;和精神健康。RUCDR为超过87个NIH资助的研究复杂疾病的受资助者提供临床生物样本和数据处理以及各种存储服务，这些疾病被认为具有遗传或表观遗传病因学成分，包括自闭症，精神分裂症，双相情感障碍，阿片类药物滥用，尼古丁成瘾，酒精中毒，炎症性肠病，肝脏疾病和肾脏疾病。RUCDR保存了超过200，000个个体生物样本，大多数是由RUCDR从NIH研究人员提交的受试者血液样本中建立的淋巴母细胞系。此外，在过去的5年中，RUCDR已经向NIH批准的研究人员分发了60多万份DNA样本。几倍增加的样本数量从更多的国家卫生研究院的项目，RUCDR现在必须支持和扩大的范围的国家最先进的分子生物学服务，RUCDR现在提供了严重的空间短缺，基础设施不足，样本安全和工作流程的问题。为了应对这些挑战，RUCDR不得不借用现有的空间，这些空间不适合RUCDR的活动，分散在三个不同的建筑物中。然而，这些空间必须在不久的将来腾出供非RUCDR使用。RUCDR现在需要任务设计，易于访问和连续的实验室空间，目前不存在于一个单一的区域。我们建议对纳尔逊生物实验室（NBL）C翼二楼的伦敦东区进行改造，以创建自动化（机器人）核酸提取、分配和分子分析设施。二楼C翼的伦敦西区将为RUCDR及其研究小组的计算和统计遗传学教师提供互动办公室和工作空间。这两个区域都在现有RUCDR实验室和NBL一楼C翼行政空间的正上方一层。我们还建议：（1）扩建附件II建筑，为液氮冷冻保存的活人类细胞提供更安全、更有效的备用储存场所;（2）扩建现有的真空夹套，（3）扩建现有的会议室/持续教育室，以应付日常工作-面向现有员工和新员工的会议，他们的工作将通过这次改造创造。

DESCRIPTION (provided by applicant): We request funds to renovate and improve existing spaces to provide lab and office areas for the Rutgers University Cell and DNA Repository (RUCDR), the designated core biosample processing and repository facility of four NIH Institutes; Alcohol and Alcohol Abuse; Digestive, Diabetes and Kidney Diseases; Drug Abuse; and Mental Health. RUCDR provides clinical biosample and data processing and varied repository services to more than 87 NIH-funded grantees researching complex diseases believed to have genetic or epigenetic etiological components, including autism, schizophrenia, bipolar disorder, opiate abuse, nicotine addiction, alcoholism, inflammatory bowel disease, liver disorders, and kidney diseases. RUCDR maintains more than 200,000 individual biosamples, most as lymphoblastoid cell lines established by the RUCDR from subject blood samples submitted by NIH researchers. Also, during the last 5 years, RUCDR has distributed more than 600,000 DNA samples to NIH-approved researchers. Several-fold increases in sample numbers from greater numbers of NIH projects that RUCDR must now support and the broadening of the scope of the state-of-the art molecular biology services that RUCDR now provides has created severe space shortages, infrastructural inadequacies, and sample security and workflow issues. In response to these challenges RUCDR has been forced to borrow existing spaces that are not well suited to RUCDR activities and that are dispersed over three different buildings. However, these spaces must be vacated for non-RUCDR uses in the near future. RUCDR now requires task-designed, easily accessible and contiguous laboratory space that does not currently exist within a single area. We propose renovation of the east end of the 2nd floor of C Wing, Nelson Biology Laboratories (NBL) to create facilities for automated (robotic) nucleic acid extraction, distribution and molecular analyses. The west end of the 2nd floor C Wing will provide interactive offices and work spaces for computational and statistical genetics faculty members affiliated with RUCDR and their research groups. Both areas are one floor directly above the existing RUCDR lab and administrative space in 1st floor C wing NBL. We also propose (1) additions to the Annex II building to produce a more secure and efficient back-up storage site for viable human cells cryopreserved in liquid nitrogen, (2) extension of the existing vacuum-jacketed, stainless steel liquid nitrogen delivery piping in Annex I to accommodate greater numbers of storage tanks and (3) expansion of the existing conference/continuing education room to accommodate daily task-oriented meetings of existing employees and new hires whose jobs will be created through this renovation.