Assembly of the 30S ribosomal subunit

描述(由申请人提供)：此申请概述了由首席研究员(威廉姆森)与俄罗斯普什基诺细胞生物物理研究所的Alexey Karnaukhov博士开展的合作研究计划。母基金是GM-53757，“SOS核糖体亚单位的组装”。母基金的总体目标是研究SOS核糖体的组装机制，SOS核糖体由一个大的16S核糖体RNA和20个小蛋白质组成。SOS亚基可以在体外重组，父母赠款的主要目标之一是研究这一过程的动力学。发展了一种同位素脉冲追踪法，可以同时监测所有20种蛋白质的结合率。动力学极其复杂，既有平行结合，也有顺序结合。生成的中间体很多，反应动力学极其复杂。FIRCA应用程序的目标是与卡诺霍夫博士合作，卡诺霍夫博士是分析非线性动态数据的专家。他开发了新的分析方法，可以直接应用于核糖体组装问题。我们已经启动了一项合作，根据这一共同兴趣开发新的工具，用于分析复杂的动力学数据。这个应用程序试图支持这项有趣而重要的工作。拟议的工作对核糖体组装问题特别重要，而且更具普遍意义，因为我们开发的工具将在了解化学、生物化学和细胞代谢的复杂反应动力学方面具有广泛的适用性。这项研究将主要在俄罗斯的俄罗斯科学院进行，与詹姆斯·R·威廉姆森博士合作，作为NIH补助金*R01 GM053757的延伸。

DESCRIPTION (provided by applicant): This application outlines a collaborative research plan to be carried out by the Principal Investigator (Williamson) with Dr. Alexey Karnaukhov at the Institute of Cell Biophysics in Pushchino, Russia. The parent grant is GM-53757, "Assembly of the SOS ribosomal subunit". The overall goal of the parent grant is to study the mechanism of assembly of the SOS ribosome, which is composed of a large 16S ribosomal RNA, and 20 small proteins. The SOS subunit can be reconstituted in vitro, and one of the major goals of the parent grant is to study the kinetics of the process. An isotope pulse chase assay has been developed that allows monitoring of binding rate of all 20 proteins simultaneously. The kinetics are extremely complex, and both parallel and sequential binding is observed. There are many intermediates formed, and the kinetics are extremely complex. The goal of the FIRCA application is to collaborate with Dr. Karnaukhov, who is an expert at the analysis of nonlinear dynamic data. He has developed new analytical approaches that can be directly applied to the problem of ribosome assembly. We have already initiated a collaboration to develop new tools for the analysis of complex kinetic data based on this common interest. This application seeks to support this interesting and important work. The proposed work is specifically significant for the ribosome assembly problem, and is more generally significant because the tools we develop will have broad applicability in understanding complex reaction dynamics in chemistry, biochemistry, and cell metabolism. The research will be done primarily in Russia at the Russian Academy of Sciences, in collaboration with Dr. James R. Williamson as an extension of NIH grant* R01 GM053757.