Microsphere Coatings for Immunoassays

描述（由申请人提供）：该项目旨在开发和展示一种用于诊断，生物技术和治疗用途的微/纳米颗粒的简便，具有成本效益和广泛适用的薄膜涂层技术。这种方法需要制备和使用在疏水嵌段中含有可光活化的偶联和聚合基团以及在亲水嵌段中含有钝化和生物分子偶联基团的两亲性嵌段共聚物。目前的商业微粒缺乏用于基因组学、蛋白质组学和细胞组学测定系统的流动颗粒和固定化微球阵列所需的表面性质的容易提供;用于细胞组学和组织工程应用的涂覆的纳米纤维;用于自编码颗粒制备和用于需要活细胞摄取颗粒的疾病研究和治疗的涂覆的“量子点”。在第一阶段，将开发新的PhotoLink试剂和涂层技术，用于在体外和体内诊断用途的微米和纳米颗粒上共价结合自组装单层膜。预期在生物分子固定效率、稳定性和比活性的性质方面比当前商业微球产品至少改善50%。该计划提出了一种创新的组合，在两亲性嵌段共聚物设计的自组装单层涂层与光活化的双自由基固定和交联化学，亲水性“刷”钝化涂层，生物分子的固定和稳定性能。

DESCRIPTION (provided by applicant): This project is designed to develop and demonstrate a facile, cost-effective, and broadly applicable thin-film coating technology for micro/nanoparticles in diagnostic, biotech, and therapeutic uses. This approach requires the preparation and use of amphiphilic block copolymers containing photoactivatable coupling and polymerizing groups in the hydrophobic block and passivating and biomolecule coupling groups in the hydrophilic block. Current commercial microparticles lack the facile provision of surface properties needed for flowing particle and immobilized microsphere arrays for genomic, proteomic and cellomic assay systems; coated nanofibers for cellomic and tissue engineering applications; coated "quantum dots" for self-encoded particle preparation and for disease studies and treatment requiring particle uptake by living cells. In Phase I, new PhotoLink reagents and coating technology will be developed for covalent bonding of self-assembling monolayer film on micro- and nanoparticles for in vitro and in vivo diagnostic uses. A 50% minimum improvement over current commercial microsphere products is expected in the properties of biomolecule immobilization efficiency, stability, and specific activity. This plan presents an innovative combination in amphiphilic block copolymer design of self-assembling monolayer coating with photoactivatable diradical immobilization and crosslinking chemistry, hydrophilic "brush" passivating coating, and biomolecule immobilization and stabilization properties.