Core--Cell culture

描述(由申请人提供) 参与该计划项目拨款的研究人员因细胞利益而团结在一起 心脏对缺血再灌注反应的潜在机制。细胞培养核心单元将通过提供每个项目所需的分离的心肌细胞和成纤维细胞来促进研究计划的目标，以研究G蛋白偶联受体调节、细胞内信号和线粒体功能。该核心还将为每个项目进行特定的实验，并容纳缺氧-复氧体外研究所需的设备和技术专业知识。

DESCRIPTION (provided by applicant) The investigators participating in this Program Project Grant are united by interests in the cellular mechanisms underlying the heart.s response to ischemia-reperfusion. The Cell Culture Core Unit will promote the objectives of the research program by providing isolated cardiac myocytes and fibroblasts required by each Project for investigation of G protein-coupled receptor regulation, intracellular signaling, and mitochondrial function. This Core will also performed specific experimentation for each Project and house the equipment and technical expertise required for in vitro studies of hypoxia-reoxygenation.