Effect of Green Tea Catechins on Tumor Vascularization

描述（由申请人提供）：绿色茶衍生的儿茶素，特别是（-）-表没食子儿茶素-3-没食子酸酯（EGCG）已显示通过包括抑制肿瘤新血管形成的几种机制具有抗肿瘤作用。肿瘤进展需要血管网络的发展来提供氧气和营养。随着血管网络的发展，其由局部血管的选择和骨髓（BM）衍生的内皮祖细胞（EPC）的浸润两者促成。我们的长期目标是开发将补充和替代药物（CAM）与标准疗法相结合的策略，通过破坏EPC对肿瘤新血管形成的贡献来阻止肿瘤进展。我们设计了一种鼠模型，以实现以下特定目标： 1.为了确定在鼠可移植培养的肿瘤模型中，儿茶素对肿瘤脉管系统形成的抑制是否是由来自相邻预先存在的血管的内皮细胞浸润肿瘤的失败引起的，或者是阻断骨髓来源的内皮祖细胞运输到肿瘤的结果。 2.探讨儿茶素对原发性和复发性乳腺癌血管发育的影响。

DESCRIPTION (provided by applicant): Green tea derived catechins, specifically (-)-epigallocatechin-3-gallate (EGCG) have been shown to have anti-tumor effects through several mechanisms including inhibiting development of the tumor neovasculature. Tumor progression requires development of a vascular network to provide oxygen and nutrients. As the vascular network develops it is contributed to by both co-opting of local blood vessels and infiltration of bone marrow (BM) derived endothelial progenitor cells (EPC). Our long-term goal of developing strategies combining complementary and alternative medicines (CAM) and standard therapies to arrest tumor progression by disrupting the contribution of EPC to the developing tumor neovasculature. We have designed a murine model to accomplish the following specific aims: 1. To determine in a murine transplantable cultured tumor model, whether the inhibition of tumor vasculature formation by catechins is caused by the failure of endothelial cells from adjacent pre-existing vessels to infiltrate the tumor or is a result of blocking bone marrow derived endothelial progenitors trafficking to the tumor. 2. To study the effects of catechins on the developing vasculature in primary and recurrent mammary carcinoma.