Mussel-Inspired Protective Coatings

描述(由申请人提供)：贻贝产生外化的肌腱，称为肌腱线，这些肌腱共同充当禁食。双面丝线覆盖有一层薄薄的保护性角质层(5-20QM)，可防止沙子、化学侵蚀和微生物降解的磨损，但具有足够的灵活性，可以容纳纤维状角质层核心的0.7应变。MFP-1是该涂层的主要结构蛋白。它还被用作一种通用的细胞和组织附着因子，并正在被开发为一种仿生系绳，用于聚合物在表面的附着。本建议旨在更好地了解MFP-1在粉末涂料中的配置、性质和工艺。这被认为是生物医学材料应用中智能合成和使用类似物所必需的。研究目标包括1)MFP-1在涂层和足部斑驳颗粒中的免疫定位；2)将元素组成与涂层的纳米机械性能进行映射；使用电化学螯合标尺测量四种不同贻贝(紫贻贝、贝壳贻贝、紫贻贝和七彩贻贝)的FP-1与金属的结合亲和力；以及4)以耗散模式通过石英晶体微天平分析研究金属诱导吸附的FP-LS的崩塌动力学。在第三年和第四年，将通过溶剂挥发、凝聚和受控自组装过程在体外制备由添加金属和不添加金属的MFP-1组成的仿生涂层。所有的薄膜都将通过宏观和纳米机械测试进行评估，并将检查它们对各种生化、热和机械损伤的耐受性。

DESCRIPTION (provided by applicant): Mussels produce externalized tendons called byssal threads, which collectively serve as holdfasts. Byssal threads are coated with a thin protective cuticle (5-20 qm) that shields against abrasion by sand, chemical erosion, and microbial degradation, yet is flexible enough to accommodate strains of 0.7 by the fibrous byssal core. Mfp-1 is the primary structural protein of the byssal coating. It is also used commercially as a universal cell and tissue attachment factor and is being developed as a biomimetic tether for polymer attachment to surfaces. The present proposal aims to better understand the disposition, properties, and processing of mfp-1 in the byssal coating. This is considered essential for the intelligent synthesis and use of analogues for biomedical materials applications. Research aims include 1) the immunolocalization of mfp-1 in the mottled granules of the coating and foot; 2) mapping the elemental composition with the nanomechanical properties of the coating; measurement of metal binding affinities of fp-ls from four different mussel species (Mytilus galloprovincialis, M. californianus, Modiolus modiolus and Septifer bifurcatus) using an electrochemical chelate scale, and 4) investigating metal-induced collapse kinetics in adsorbed fp-ls by quartz crystal microbalance analysis in dissipation mode. In years 3 and 4, biomimetic coatings comprised of mfp-1 with and without added metal will be fabricated in vitro by solvent evaporation, coacervation, and controlled self-assembly processes. All films will be evaluated by macro- and nanomechanical tests, and their resistance to a diverse range of biochemical, thermal, and mechanical insults will be examined.