3D Digital Modeling of the Drosphila Brain

描述(申请人提供)：数字3D模型在神经科学中发挥着越来越重要的作用。数字模型代表了功能数据和基因表达数据被输入并以图形显示的三维支架，成为允许人们解决神经连接和功能以及基因功能和基因相互作用的分析工具。这项拨款申请计划为发育中的果蝇大脑生成一系列标准化的数字图谱模型，许多人使用该系统来研究控制神经元电路形成和功能的遗传机制。苍蝇脑是由一组不变的神经母细胞谱系组成的，这些谱系代表了细胞体位置、轴突投射和(在本提案中将讨论的程度)连接性方面的结构单位。轴突和树突的分支，建立了从胚胎晚期到成体可见的形态上不同的神经堆室。隔间和谱系形成了一种刻板印象的模式，将在拟议的数字模型中捕捉到。考虑到它们对我们和其他人的实用性以及可行性，提出了以下模型：(1)早期胚胎神经母细胞图谱，(2)与进化的神经堆有关的晚期胚胎初级谱系，(3)与神经堆室有关的晚期幼虫次级谱系，(4)发育中的次级束系统和蛹的神经堆室。这些模型代表了一个完整的系列，因为每个神经母细胞的“遗传地址”，由早期胚胎神经母细胞图谱中表达的已知基因集定义，将与为晚期胚胎、幼虫和幼虫建模的神经元及其轴突群体联系在一起。这个建模项目的目标是提供一个与社区共享的工具，允许更有效地利用Drosphila大脑来解决发育-遗传学和功能性问题。神经谱系的模型将使对特定实验进行阶段化和解释突变表型成为可能。

DESCRIPTION (provided by applicant): Digital 3D models play an increasingly important role in neuroscience. Representing three-dimensional scaffolds in which functional data and gene expression data are entered and displayed graphically, the digital models become analytical tools that allow one to address neural connectivity and function, as well as gene function and gene interactions. This grant application proposes to generate a series of standardized digital atlas models of the developing Drosophila brain, a system used by many to investigate the genetic mechanism controlling the formation and function of neuronal circuits. The fly brain is formed by an invariant set of neuroblast lineages which represent structural units in terms of cell body location, axonal projection, and (to an extent that will be addressed in this proposal) connectivity. Axonal and dendritic arborizations, establish morphologically distinct neuropile compartments that are visible from the late embryo towards the adult. Compartments and lineages form a stereotyped pattern that will be captured in the proposed digital models. Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. These models represent an integrated series because the "genetic address" of each neuroblast, defined by the known sets of genes expressed in the early embryonic neuroblast map, will be linked to the population of neurons and their axons modeled for the late embryo, larva and pupa. The goal of this modeling project is to provide a tool shared with the community, allowing to exploit the Drosphila brain more efficiently for developmental-genetic and functional questions. Models of neural lineages will make it possible to phrase specific experiments and to interpret mutant phenotypes.