IMMUNE RESPONSES IN HEALTHY ADULTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。浆母细胞是产生抗体的细胞的前体，抗体是对抗感染所必需的，但如果产生不当，也会导致疾病。浆母细胞是在正常的免疫反应中产生的，但在人类中驱动这种反应的机制尚不清楚。系统性红斑狼疮（SLE）中浆母细胞异常产生。SLE还与BLyS和1型干扰素（IFN-I）水平升高相关，这可能诱导成浆细胞分化。血清BLyS或IFN-1的增加是否在正常免疫应答中可检测到，而不是SLE中的异常自身免疫应答，尚不清楚。本研究的主要目的是确定在健康个体的正常免疫应答期间，是否可在血液中检测到BLyS或IFN-1的增加。 这项初步研究于第1年完成，使用GCRC对10名健康受试者进行了pneumovax给药。将在第0、3、6和9天采集血样。Carter博士使用这些样本研究浆母细胞、IFN-1和BLyS。 初步研究表明，在正常的免疫应答中，血清IFN-1升高。该信息将用于研究第二组15名健康受试者和15名狼疮受试者，以确定健康个体和狼疮受试者之间正常免疫应答中BLyS和IFN-1应答的变异性。这将确定BLyS和/或IFN- 1的增加是否在SLE患者中固有地更大，这是这种疾病的潜在机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Plasmablasts are the precursors of the cells that produce antibodies, which are necessary to fight infections but which can also cause disease when produced inappropriately. Plasmablasts are generated in a normal immune response, but the mechanisms driving this response are poorly defined in humans. Plasmablasts are produced abnormally in systemic lupus erythematosus (SLE). SLE is also associated with increased levels of BLyS and type-1 interferons (IFN-I), which may induce plasmablast differentiation. Whether increases in serum BLyS or IFN-1 are detectable in a normal immune response, as opposed to the abnormal, autoimmune response in SLE, is unknown. The primary goal of this study is to determine whether increases in BLyS or IFN-1 are detectable in the blood during a normal immune response in healthy individuals. This preliminary study, completed in year 1, used the GCRC to administer pneumovax to 10 healthy subjects. Blood samples will be taken at days 0, 3, 6, and 9. Dr. Carter used the samples to study plasmablasts, IFN-1 and BLyS. The preliminary study demonstrated an increase in serum IFN-1 in normal immune response. This information will be used to study a second cohort of 15 healthy subjects and 15 with lupus, to define the variabilty in BLyS and IFN-1 responses in normal immune responses between healthy individuals and those with lupus. This will determine whether the increase in BLyS and/or IFN- 1 are inherently greater in SLE patients, a potential mechanism for this disease.