Analytic and Preparative Cytometry Facility

分析和制备流式细胞术核心 (APFCC) 旨在增强功率和 通过提供最新的数据，提高风湿病核心中心 (RDCC) 研究人员的研究生产力 以合理的成本进行细胞分析和纯化的流式细胞术。为了实现这一目标， Core 提供流式细胞术应用所需的设备、服务和专业知识， 风湿病前沿研究的相关技术。我们使用了国家同行评审的 联邦仪器补助金和 UAB 资源，以获得顶级设备，提供 为 RDCC 研究人员提供广泛的应用。其中包括 20 个检测器、4 激光分析细胞仪 （2006 年获得）、一台 14 个检测器、3 个激光、一台增强型全数字分选细胞仪（2006 年升级）、一台 6 个探测器 检测器、配备装载机的分析仪和 5 检测器分析仪。此外，核心还提供设备 辅助技术，例如自动样品制备（2005 年收购）、荧光光谱法和 自动磁力富集（2005 年收购）。我们开发了新技术并提供了 基本样本分析，结果表明 APFCC 已为 120 多个独立资助的 UAB 提供服务 研究人员并为许多出版物做出了贡献。我们的核心目标是继续努力进行外展 和教育，提醒 RDCC 调查人员注意流动技术增强其能力的潜力 研究、服务，以提高效率并保持尽可能高的质量标准，以及 应用程序开发，继续为 RDCC 研究带来新技术和协议 社区。为了实现这些目标，需要资金支持外展、教育、质量控制 核心总监和核心经理的开发活动，并部分抵消其中一名成员的成本 服务合同，以保持不间断地访问这一重要技术。科学咨询委员会， 它结合了知识渊博的用户和参与 UAB 高级研究职位的人员， 外部顾问，他们非常了解更新的设备和应用程序，以及与 更大的 UAB 机构将提供反馈并推动进一步发展。通过这些努力，我们将 保持流式细胞术作为风湿病最先进研究的基本、重要工具 阿拉巴马大学。

The Analytic and Preparative Flow Cytometry Core (APFCC) is designed to enhance the power and productivity of the research of Rheumatic Disease Core Center (RDCC) investigators by providing state-ofthe- art flow cytometry for cell analyses and purification at a reasonable cost. To accomplish this goal, the Core provides the equipment, service and expertise necessary for the application of flow cytometry and related technologies to cutting edge research in the rheumatic diseases. We have used nationally peerreviewed federal instrumentation grants and UAB resources to obtain top-of-the-line equipment that provides a wide range of applications for RDCC investigators. This includes a 20-detector, 4-laser analytic cytometer (obtained 2006), a 14-detector, 3-laser, a sort enhanced all digital sorting cytometer (upgraded 2006), a 6- detector, loader equipped analyzer, and a 5-detector analyzer. In addition, the core provides equipment for ancillary techniques such as automated sample preparation (acquired 2005), spectrofluorimetry, and automated magnetic enrichment (acquired 2005). We have developed new techniques as well as provided basic sample analysis, with the result that the APFCC has served over 120 independently funded UAB investigators and contributed to many publications. Our goals for the core are to continue efforts at outreach and education, to alert RDCC investigators to the potential for flow technology to increase the power of their research, service, to improve efficiency and to maintain the highest possible standards of quality, and application development, to continue to bring new technologies and protocols to the RDCC research community. To achieve these goals, funds are requested to support outreach, educational, quality control and development activities of the Core Director and Core Manager and partially offset the costs of one service contract to maintain uninterrupted access to this vital technology. The-Scientific Advisory Committee, which combines both knowledgeable users and those involved in senior research positions at UAB, an external advisory, who is well informed about newer equipment and applications, and an integration with the larger UAB institution will provide feedback and drive further development. With these efforts, we will maintain flow cytometry as a fundamental, vital tool for state-of-the-art research in the rheumatic diseases at UAB.