FLOW CYTOMETRY & HIGH SPEED CELL SORTING: HIV GP120, HIV-1 INFECTION

流式细胞术

• 批准号: 7335196
• 负责人: Robert H Carter
• 金额: $ 4.41万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335196
• 关键词: flow cytometry; infection; lasers

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): The UAB Flow Cytometry and High-Speed Cell Sorting Facility provides expert assistance and instruments for analysis of particles and cells that is available to all NIH-funded researchers at UAB. The current equipment in the core includes a FACS Vantage (since upgraded to DiVa), a FACS Calibur and a FACScan, which were purchased with support from both the NIH and the institution. These cytometers have limited capabilities in the number and type of fluorochromes and fluorescent particles they can detect. In this proposal, twenty-three NIH-funded, UAB investigators request funds for purchase of a BD LSR II cytometer. The LSR II will be equipped with a solid-state 488nm blue laser (20mw) a JDS Uniphase 633nm Red laser (18mw) and 405nm Violet laser (25mw) and a 532nm Green laser (Variable 0-150 mw). The system will have the ability to detect 2 colors from the blue laser plus side and forward scatter utilizing a trigon optical array, 3 colors from the red laser utilizing a trigon optical array, 8 colors off the violet laser utilizing an octagon optical array and 5 colors off the green laser utilizing an octagon optical array. The system is capable of collecting up to 20 parameters. The collected emission light will be delivered to the optical arrays by fiber optic technology. Appropriate bandpass filters and dichroic mirrors are included. The LSR II cytometer will provide access to three technologies not currently available to UAB researchers: Multi-parameter analysis of a wide spectrum of fluorochromes; Excitation and detection of emission for multiple reporter constructs; Excitation and detection of fluorescent emission from quantum dots. This proposal documents the experimental plans of the co-investigators for use of the expanded technical capabilities, the absence of accessible alternatives, and the institutional administrative and material support for this cytometer. Combined with efforts by the Facility in investigator education and outreach, this proposal will significantly improve the flow cytometry services in support of NIH funded projects at UAB.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。关于艾滋病描述（由申请人提供）：UAB 流式细胞术和高速细胞分选设施为颗粒和细胞分析提供专家帮助和仪器，供 UAB 的所有 NIH 资助的研究人员使用。目前核心设备包括 FACS Vantage（现已升级为 DiVa）、FACS Calibur 和 FACScan，这些设备是在 NIH 和该机构的支持下购买的。这些细胞仪在可检测的荧光染料和荧光颗粒的数量和类型方面能力有限。在此提案中，23 名 NIH 资助的 UAB 研究人员请求资金购买 BD LSR II 细胞仪。 LSR II 将配备固态 488nm 蓝色激光器（20mw）、JDS Uniphase 633nm 红色激光器（18mw）和 405nm 紫罗兰激光器（25mw）以及 532nm 绿色激光器（可变 0-150 mw）。该系统将能够利用三角光学阵列检测来自蓝色激光正侧和前向散射的 2 种颜色，利用三角光学阵列检测来自红色激光的 3 种颜色，利用八边形光学阵列检测来自紫色激光的 8 种颜色，利用八边形光学阵列检测来自绿色激光的 5 种颜色。该系统能够收集多达 20 个参数。收集到的发射光将通过光纤技术传送到光学阵列。包括适当的带通滤光片和二向色镜。 LSR II 细胞仪将提供 UAB 研究人员目前无法使用的三种技术： 多种荧光染料的多参数分析；多个报告基因构建体的激发和发射检测；量子点荧光发射的激发和检测。该提案记录了联合研究人员利用扩展的技术能力、缺乏可用替代方案以及该细胞仪的机构管理和物质支持的实验计划。结合该机构在研究者教育和推广方面的努力，该提案将显着改善流式细胞术服务，以支持 NIH 资助的 UAB 项目。