Malaria Parasite Receptors And Host Cell Ligands

目的：1.鉴定疟原虫红细胞侵入受体和感染红细胞粘附于内皮和胎盘的受体。2.确定每种寄生虫受体的红细胞配体。3.鉴定与胎盘结合的变异抗原的最小结构域。4.测试变异抗原上的一个区域，用于诱导Aotus猴对恶性疟原虫的保护和克服变异。 本年度取得的成绩：1。BAEBL配体被纯化的血型糖蛋白C阻断，但不被N-聚糖酶处理的血型糖蛋白C阻断。与Gerbich红细胞相比，正常红细胞血型糖蛋白C上的N-聚糖不同2。已鉴定出AMA1结合红细胞的受体结构域以及红细胞上结合AMA1的Kx蛋白。3.已经确定了Dd2（神经氨酸酶处理的红细胞无侵入）和Dd2/nm（神经氨酸酶处理的红细胞侵入）之间的差异。

Purpose: 1. To identify the receptors on malaria parasites for red cell invasion and on infected red cells for adhesion to endothelium and to placenta. 2. To identify the red cell ligands for each parasite receptor. 3. To identify the minimal domain in the variant antigen for binding to placenta. 4. Test a region on the variant antigen for inducing protection against P. falciparum in Aotus monkeys and for overcoming variation. Accomplishments during the year: 1. The BAEBL ligand is blocked by purified glycophorin C, but not by N-glycanase treated glycophorin C. The N-glycan on glycophorin C differs for normal red cells compared to Gerbich red cells 2. The receptor domain of AMA1 for binding red cells has been identified as well as the Kx protein on the red cell for binding of AMA1. 3. The difference between Dd2 (no invasion of neuraminidase treated red cells) and Dd2/nm (invasion of neuraminidase treated red cells) has been identified.