Fluorescent activated cell sorter for UNTHSC

描述(由申请人提供)：这项提案要求为最先进的荧光激活细胞分类器Beckman Coulter EPics Altra提供资金。该仪器将服务于位于沃斯堡的北德克萨斯大学健康科学中心越来越多的研究人员，以及来自当地机构的其他一些研究人员。目前在UNTHSC或Fort Worth没有细胞分选器。用户必须前往达拉斯的德克萨斯大学西南医学中心对细胞进行分类。这限制了研究生使用这种强大的方法获取数据的能力。所要求的仪器将存放在分子生物学和免疫学系卫生科学中心的流式细胞术核心。Beckman Coulter EPICS Altra是一种最先进的仪器，将使北德克萨斯大学健康科学中心能够满足其研究人员当前和未来的需求，并使其教学技术现代化。利用更广泛的荧光染料和对稀有细胞进行高速分选的能力将增加可用于解决重要研究问题的方法的类型。所有主要用户都至少拥有一项NIH同行评审拨款。因此，该文书将对美国国立卫生研究院资助的几个项目产生重大益处，并使提出的一系列问题能够继续取得进展。

DESCRIPTION (provided by applicant): This proposal requests funding for a state-of-the-art fluorescence activated cell sorter, the Beckman Coulter Epics Altra. This instrument will serve a growing community of researchers at the University of North Texas Health Science Center at Fort Worth as well as a number of other researchers from local institutions. There is currently no cell sorter in UNTHSC or Fort Worth. Users have to travel to the University of Texas Southwestern Medical Center in Dallas to sort cells. This limits the ability of graduate students to obtain data using this powerful approach. The requested instrument will be housed in the Flow Cytometry Core of the Health Science Center within the Department of Molecular Biology and Immunology. The Beckman Coulter Epics Altra is a state-of-the-art instrument that will enable the University of North Texas Health Science Center to meet the current and future needs of its researchers, as well as modernize its teaching technology. The ability to utilize a wider range of fluorochromes and perform high speed sorting of rare cells will increase the types of approaches that can be used to address important research questions. All of the major users hold at least one NIH peer reviewed grant. Thus, the instrument will be of significant benefit for several NIH funded projects and enable continued progress to a wide range of questions being asked.