REGULATION OF OVARIAN SURFACE EPITHELIAL CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。卵巢表面上皮（OSE）是卵巢周围的单层，占卵巢肿块的不到千分之一，但它导致了近90%的女性卵巢癌。一生中患卵巢癌的风险几乎是1 / 50，OSE细胞必须被认为是最容易转化的细胞之一。目前还没有针对这些卵巢癌的动物模型，因为它们只在灵长类动物和母鸡中被报道过。本项目旨在建立非人类灵长类动物模型，研究恒河猴OSE （RhOSE）的体内和体外细胞生物学。最初的研究建立了RhOSE细胞培养，并与人类OSE细胞进行了比较分析。这些数据显示人类和恒河猴的OSE细胞高度相似，共享在实验室或家畜（如啮齿动物、羊和猪）的OSE细胞中未见的基因表达模式。该项目的长期目标是利用细胞培养、微阵列和分子技术来确定RhOSE的增殖和存活是如何在体外调节的，并将这些发现转化为体内的整个动物。这些努力的结果可能表明哪些生理事件促进了OSE转化，并可能转化为临床应用，以开发更有效的卵巢癌治疗方法，早期发现和预防手段。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ovarian surface epithelium (OSE) is a monolayer surrounding the ovary that comprises less than 1/1,000th of the ovarian mass, yet it gives rise to nearly 90% of ovarian cancers in women. With a life-time risk of ovarian cancer almost 1 in 50, OSE cells must be considered among the most highly prone to transformation. Currently no animal model exists for these ovarian cancers, because they have only been reported in primates and the hen. This project was developed to facilitate a nonhuman primate model to investigate rhesus macaque OSE (RhOSE) cell biology in vitro and in vivo. Initial studies established RhOSE cell cultures and performed comparative analysis with human OSE cells. These data showed high similarity between human and rhesus OSE cells, sharing patterns of gene expression not seen in OSE cells from laboratory or domestic animals, such as rodents, sheep, and pigs. The long-term goal of this project is to use cell culture, microarray, and molecular techniques to determine how RhOSE proliferation and survival are regulated in vitro, and translate these findings to the whole animal in vivo. The results of these efforts may indicate what physiological events promote OSE transformation and may translate into clinical applications to develop more effective ovarian cancer therapies, means for early detection, and prevention.