ALCOHOL AND SIV ENCEPHALITIS: PBMC CHEMOTAXIS MODULATION FOLLOWING INFECTION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。猴免疫缺陷病毒（SIV）感染猕猴是获得性免疫缺陷综合征（AIDS）的重要动物模型，它概括了HIV感染的大多数特征，包括CNS疾病。酒精已被证明会加剧人类的神经系统疾病，而且众所周知，慢性酒精滥用者中艾滋病毒感染率较高。然而，目前尚不清楚这是否会导致与艾滋病毒感染相关的神经系统并发症的发生率更高。在患有晚期AIDS的猴子的脑内发现的SIV感染的PBMC的数量与疾病的严重程度相关。 为了检测SIV感染后慢性酒精滥用是否导致PBMC向CNS迁移增加，我们测定了趋化指数。这些通过测量在SIV感染之前和之后从猕猴新鲜获得的PBMC以定向方式迁移到CCL 3、4和7的能力来获得。 结果显示，在SIV感染后，PBMC以CCR 5依赖性方式向CCL 3、4和7迁移增加，并且这通过长期酒精施用而加剧。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Simian Immunodeficiency virus (SIV) infection of macaques is an important animal model for the acquired immunodeficiency syndrome (AIDS) that recapitulates most features of HIV infection, including CNS disease. Alcohol has been shown to exacerbate neurological diseases in humans, and it is known that there is a higher incidence of HIV infection among chronic alcohol abusers. However, it is not known if this translates into a higher incidence of neurological complications associated with HIV infection. Numbers of SIV infected PBMCs found within the brain of monkeys with terminal AIDS correlates with severity of disease. To examine if chronic alcohol abuse leads to increased PBMC migration into the CNS following SIV infection we determined chemotactic indices. These were obtained by measuring the ability of PBMCs freshly obtained from macaques to migrate in a directed manner to CCLs 3,4 &7 before and after SIV infection. The results showed that there is an increase in migration of PBMCs towards CCLs 3, 4 & 7 following SIV infection in a CCR5 dependent manner and that this is exacerbated by chronic alcohol administration.