Interaction of auxin and abscisic acid signaling

项目描述：本项目的长期目标是提高我们对植物激素生长素和阿坝之间的信号串扰的理解。最近克隆的IBR5，编码一个假定的双特异性磷酸酶和这个建议的主题，揭示了一个新的点之间的相互作用生长素和阿坝信号转导。有几种方法将使用IBR5来更好地了解生长素和阿坝之间的关系。首先，将分离ibr 5生长素不敏感性和阿坝不敏感性的抑制子（目标1）。将对这些抑制子进行表型表征（目的2），并克隆缺陷基因（目的3）。预期这些抑制因子中的至少一些在IBR5底物基因中携带病变。抑制子的克隆和免疫共沉淀将用于鉴定潜在的IBR 5底物（目的4）。通过研究和克隆抑制子，以及鉴定IBR5底物，将获得更深入的了解生长素和阿坝的串扰。

DESCRIPTION (provided by applicant): The long-term goal of this project is to enhance our understanding of the signaling cross-talk between the phytohormones auxin and ABA. The recent cloning of IBR5, encoding a putative dual-specificity phosphatase and the subject of this proposal, revealed a novel point of interaction between auxin and ABA signal transduction. Several approaches will use IBR5 to gain greater understanding of the relationship between auxin and ABA. Firstly, suppressors if ibr5 auxin insensitivity and ABA insensitivity will be isolated (Aim 1). These suppressors will be phenotypically characterized (Aim 2) and the defective genes will be cloned (Aim 3). At least some of these suppressors are expected to carry lesions in IBR5 substrate genes. Both cloning of suppressors and co-immunoprecipitation will be used to identify potential IBR5 substrates (Aim 4). By studying and cloning suppresors, as well as identifying IBR5 substrates, a more in-depth understanding of auxin and ABA cross-talk will be gained.