LASER SCANNING CYTOMETER

描述（由申请人提供）：本申请申请资助一种CompuCyte激光扫描细胞仪，用于定量分析固定和活细胞和组织中的荧光。激光扫描细胞仪将成为威斯康星大学综合癌症中心的流式细胞仪设备的一部分，并将服务于整个威斯康星大学的研究社区。该设施将为威斯康星大学校园内多个部门的研究人员提供服务，他们需要该仪器来定量使用活体和固定样本的同质和异质细胞群体的荧光。主要用户将需要CompuCyte激光扫描细胞仪(1)提供贴壁细胞的荧光定量和(2)分析混合群体或组织中的异质细胞群体。所有主要用户都有荧光显微镜和流式细胞术分析的经验，并确定威斯康星大学目前可用的设备不能满足他们的需求。激光扫描细胞仪将安装在位于PI实验室附近的流式细胞仪设备实验室中，位于其他研究人员的中心位置。有流式细胞术经验的人员将配备该设施，威斯康星大学综合癌症中心将为该设施提供资金支持。总之，该应用程序将满足美国国立卫生研究院（nih）资助的威斯康星大学（University of Wisconsin）科学社区的广泛需求，并将为当前可用的荧光分析技术提供重大进步。

DESCRIPTION (provided by applicant): This application requests funding for a CompuCyte laser scanning cytometer optimized for quantitative analysis of fluorescence in fixed and living cells and tissues. The laser scanning cytometer will become part of the University of Wisconsin Comprehensive Cancer Center's flow cytometry facility, and will serve the entire University of Wisconsin research community. The facility will serve investigators from multiple departments throughout the University of Wisconsin campus who need the instrument to quantify fluorescence in homogenous and heterogeneous cell populations with both live and fixed samples. The major users will require the CompuCyte laser scanning cytometer to (1) provide quantification of fluorescence in adherent cells and (2) analyze heterogeneous populations of cells within mixed populations or tissues. All major users have experience in fluorescence microscopy and flow cytometry analysis and have determined that the equipment currently available at the University of Wisconsin cannot meet their needs. The laser scanning cytometer will be installed in a flow cytometry facility laboratory located near the laboratory of the PI at a location central to the other investigators. Personnel experienced in flow cytometry will staff the facility and financial support for the facility will be provided by the University of Wisconsin Comprehensive Cancer Center. In summary, the application will serve the needs of a broad NIH-funded scientific community at the University of Wisconsin campus and will provide a significant advance over the current technology available for fluorescence analysis.