LTQ Linear Ion Trap Mass Spectrometer System

描述（由申请人提供）：在Susan Weintraub博士的指导下，申请资金为位于圣安东尼奥的德克萨斯大学健康科学中心的机构质谱实验室（MSL）购买Thermo Finnigan LTQ线性离子阱质谱仪，附带微型HPLC系统和计算机集群，Susan Weintraub博士在生物医学质谱方面拥有30多年的经验。目前，毛细管HPLC-ESI/ MS/MS分析是在1997年安装的Thermo Finnigan LCQ-Classic上进行的，MALDI-TOF质谱是在Applied Biosystems Voyager-DE STR上获得的。2003年的用户组包括36个独立的教员从14个部门在UTHSCSA除了一些研究人员从其他机构在得克萨斯州和其他地方在美国的几个技术限制的LCQ经典沿着关注其操作寿命促使努力寻求资金的MSL的一个新的工具。关于仪器的性能，提高检测极限、扫描速度和样品处理量将使大部分用户受益。LTQ在日常维护方面的优势将提高日常使用的效率。Weintraub博士在2004年2月前往圣何塞期间评价了LTQ，当时她分析了在其实验室制备的代表性样品。结果清楚地证明了获得LTQ在以下领域对MSL用户组的显著益处：（1）鉴定凝胶中的低水平蛋白质;（2）鉴定凝胶中复杂混合物中的低水平蛋白质;和（3）检测低化学计量的磷酸肽。本报告介绍了9个主要用户、4个次要用户和3个未来用户的项目说明。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Thermo Finnigan LTQ linear ion trap mass spectrometer, accompanying micro HPLC system and computer cluster for the Institutional Mass Spectrometry Laboratory (MSL) of the University of Texas Health Science Center at San Antonio, under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. At the present time, capillary HPLC-ESI/ MS/MS analyses are conducted on a Thermo Finnigan LCQ-Classic that was installed in 1997, and MALDI-TOF mass spectra are acquired on an Applied Biosystems Voyager-DE STR. These instruments have been extraordinarily powerful tools for identification and characterization of proteins and peptides for a large number of investigators. The 2003 user group consisted of 36 individual faculty members from 14 departments at UTHSCSA in addition to a number of researchers from other institutions in Texas and elsewhere in the U.S. Several technological limitations of the LCQ-Classic along with concerns about its operating lifetime prompted efforts to seek funding for a new instrument for the MSL. Regarding the performance of the instrument, improvements in detection limits, scan speed and sample through-put would benefit a large proportion of the user group. Advantages of the LTQ related to routine maintenance would enhance efficiency of day-to-day usage. Dr. Weintraub evaluated the LTQ during a trip to San Jose in February 2004 at which time she analyzed representative samples prepared in her laboratory. The results clearly demonstrated the significant benefits to the user group of the MSL that will result from acquisition of an LTQ in the following areas: (1) identification of low levels of protein in a gel; (2) identification of a low level of protein in a complex mixture in a gel; and (3) detection of a phosphopeptide at low stoichiometry. Project descriptions for 9 major users, 4 minor users and 3 future users are presented.