Amersham Typhoon 9410 Variable Model Imager

描述(由申请人提供)：约翰·霍普金斯大学生物系内由10名NIH资助的研究人员组成的多样化用户组，申请资金购买一台amersham 9410台风可变模式成像仪，以取代过时的1993年分子动力学SF Model 455，该模型自1993年以来一直是该系的主要主力。这款最先进的台风光学成像仪将取代我们的旧SF455系统，并在功能和最重要的可靠性方面构成对其的重大升级。该部门的调查人员都是由美国国立卫生研究院资助的，他们致力于一系列基本的细胞生物/生化相关问题，包括但不限于酵母中的钙信号；螺旋酶和细菌结合；蛋白质对转录和DNA结合的调节；葡萄糖磷酸转移酶系统；膜的组织和动力学；基于微管的马达；膜的运输；生殖细胞的发育和迁移；感觉神经元的发育；以及内吞的机制。在所要求的配置中，9410磷光成像仪将为我们不同的研究人员小组提供广泛的基本/高级功能，用于成像/定量凝胶三明治、全尺寸琼脂糖凝胶和聚丙烯酰胺凝胶、膜、微孔板和微阵列。我们的调查人员所需的这些能力在我们目前的SF455系统上缺失或存在缺陷，这是一个有限的格式系统。 在这份意见书中，一个更重要的问题与我们较旧的SF 455系统的老化不可靠有关；amersham已经停止提供服务/部件，以维持系统在合格的工作条件下。特别令人担忧的是，MD455设计采用了一种独特的、不可更换的整体计算机设计，其部件越来越难获得，这加剧了我们的担忧，即在不久的将来，该系统将变得不可修复，导致我们没有系统。另一项主要考虑涉及相当昂贵/杂乱的胶片冲洗机维护和照相化学品的安全处理/处置。因此，鉴于凝胶/印迹对我们的基础生物/生化研究的基本性质，以及部门调查人员在基因组学/蛋白质组学中使用微阵列分析的不断发展；加上对我们当前系统的可靠性和性能限制的担忧不断升级；我们正在寻求通过增加9410可变模式成像仪来升级我们的常用设施。 台风9410将被方便地放置在国防部的综合成像中心(IIC)内，进行管理和维护；它将被并入IIC现有的、完善的充值系统，以确保收回用于供应和维护的资金。

DESCRIPTION (provided by applicant): A diverse user-group of 10 NIH funded investigators, within the Department of Biology at The Johns Hopkins University, request funds to purchase an Amersham 9410 Typhoon Variable Mode Imager to replace an outmoded 1993 Molecular Dynamics SF Model 455, which has been the primary workhorse for the department since 1993. This state of- the-art Typhoon phosophorimager will replace and constitute a significant upgrade from our older SF 455 system in terms of capabilities and, most importantly, reliability. Department investigators, all well funded through the NIH, work on a host of basic cell biological/biochemical related questions including, though not limited to, calcium signaling in yeast; helicases and bacterial conjugation, regulation of transcription and DNA binding by proteins, glycose phosphotransferase system, membrane organization and dynamics; microtubule-based motors; membrane trafficking; germ cell development and migration; sensory neuron development; and mechanisms of endocytosis. In the requested configuration, the 9410 phosophorimager will afford our disparate group of investigators a broad range of basic/advance capabilities for imaging/quantifying gel sandwiches, full-sized agarose and polyacrylamide gels, membranes, microplates, and microarrays. These capabilities required by our investigators are absent or deficient on our current SF 455 system, a limited format system. An even more critical concern in this submission pertains to the age-related unreliability of our older SF 455 system; and that Amersham has discontinued supplying service/parts to maintain the system in competent working condition. Particular worrisome is that the MD 455 design incorporates a unique, un-replacable integral computer design for which parts have been progressively more difficult to acquire, reinforcing our concern that in the near future the system will become un-repairable leaving us with no system. An additional major consideration relates to the rather costly/messy maintenance of the film processor and safe handling/disposal of photographic chemicals. Consequently, given the essential nature of gels/blots to our basic biological/biochemical research and the evolving use of microarray analysis in genomics/proteomics by department investigators; coupled with escalating concerns pertaining to reliability and performance limitations of our current system; we are seeking to upgrade our common-use facilities with the addition of the 9410 variable mode imager. The Typhoon 9410 will be conveniently located, administered, and maintained, in the Department's Integrated Imaging Center (IIC); and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.