High Pressure Freezing and Processing Unit

描述(由申请人提供)： 印第安纳大学医学院的电子显微镜中心迫切需要徕卡EM PACT高压冷冻(HPF)和处理系统。HPF以每秒13,000度的速度快速冷冻显微活检、组织切片或培养细胞。自动冷冻替代处理机允许对这些标本进行统一处理。该系统代表了无需化学固定即可进行电子显微镜(EM)组织处理的最新方法。EM中心是一个致力于研究的多用户设施，也是印第安纳大学医学院的研究核心实验室。EM中心自1961年以来一直服务于印第安纳大学研究人员的研究需求，并为其研究贡献提供了学校和部门的大力支持。EM中心有一名全职教员主任加顿博士和一名全职经理米勒女士，并向所有印第安纳大学医学院的调查人员开放。目前的申请是为了徕卡高压冷冻机和处理系统，因为需要八名NIH资助的研究人员。大多数研究人员需要该系统来进行免疫细胞化学以固定敏感抗原或避免由于化学固定而可能产生的伪影。然而，一些项目有非常创新的需求，例如Molitoris博士使用徕卡针显微活组织检查系统，在活体情况下使用双光子共聚焦显微镜从大鼠肾脏中提取样本。埃文医生需要冻结直接从手术室(OR)获得的样本。徕卡HPF的移动性使该设备可以通过OR或双光子共聚焦显微镜套件轻松地运送到病理室。两位高素质的EM专家，Miller女士和Pennington女士，在徕卡冷冻和处理系统的使用和维护方面拥有专业知识。研究和物理环境、EM中心人员和印第安纳大学医学院的机构支持使IUEM中心成为适当使用徕卡HPF和处理器系统的绝佳场所。

DESCRIPTION (provided by applicant): The Electron Microscopy Center at the Indiana University School of Medicine has an urgent need for a LEICA EM PACT High Pressure Freezing (HPF) and Processing System. The HPF rapidly freezes microbiopsies, tissue pieces or cultured cells at a rate of >13,000 degrees/second. The automated freeze substitution processor allows these specimens to be uniformly processed. This system represents the newest approach to processing tissues for electron microscopy (EM) without chemical fixation. The EM Center is a multi-user facility dedicated to research and a Research Core Laboratory for the Indiana University School of Medicine. The EM Center has served the research needs of IU investigators since 1961 with strong School and Departmental support for its research contributions. The EM Center has a full-time faculty Director, Dr. Gattone, a full-time manager, Ms Miller, and is available to all IU School of Medicine investigators. The present application is for the Leica High Pressure Freezer and Processing System because of a need by eight, NIH funded investigators. Most investigators need this system to perform immunocytochemistry for fixation sensitive antigens or to avoid potential artifacts due to chemical fixation. However, some projects have very innovative needs, such as Dr. Molitoris' use of the Leica needle microbiopsy system, for samples taken from a rat kidney while viewing it, in vivo, with a two photon confocal microscope. Dr. Evan needs to freeze samples obtained directly from the operating room (OR). The mobility of the Leica HPF allows the unit to be easily transported to the pathology room by the OR or two-photon confocal microscope suite. Two highly qualified EM Specialist, Ms. Miller and Pennington, have expertise in the use and maintenance of this Leica freezing and processing system. The research and physical environment, EM Center personnel and IU School of Medicine institutional support make the IU EM Center an excellent place for the appropriate use of the Leica HPF and Processor System.