Core--Analytical glycotechnology

描述(由申请人提供)： 分析糖技术核心的目的是提供技术专业知识和 N-和O-连接的唾液酸结构表征所需的仪器 从蛋白质、细胞或组织中分离出的低聚糖或糖胺聚糖 项目负责人实验室提供转基因动物。核心将大大受益 从获得现有糖化技术中的仪器、试剂和专业知识 加州大学圣迭戈分校糖生物学研究和培训中心(GRTC)的资源核心。用于这些分析的许多技术(脂类提取、多糖分离、单糖分析、甲基化连锁分析、糖胺多糖二糖分析)都是在前一个供资期间设计或实施的。其他的只是最近才推出的，因为GRTC Core增加了新的、最先进的设备，旨在提高灵敏度。这些升级包括用于现有LC-Q质谱仪的纳米放射源、MALDI-TOF质谱仪、高灵敏度荧光探测器和用于核磁共振的新纳米探测器。高质量仪器的可获得性，加上以以下成员为代表的多年分析糖生物学经验 GRTC Glycotech资源，使我们能够应对 并进行必要的分析，以支持他们的研究目标。

DESCRIPTION (provided by applicant): The purpose of the Analytical Glycotechnology Core is to provide the technical expertise and instrumentation necessary to structurally characterize sialic acids,N- and O-linked oligosaccharides or glycosaminoglycans isolated from either proteins, cells or tissues from transgenic animals provided by the Project Leaders' laboratories. The Core will greatly benefit from access to the instruments, reagents and expertise in the existing Glycotechnology Resource Core of the UCSD Glycobiology Research and Training Center (GRTC). Many of the techniques to be employed for these analyses (lipid extraction, isolation of glycans, monosaccharide analysis, methylation linkage analysis, glycosaminoglycan disaccharide analysis) were devised or implemented during the previous funding period. Others have only recently become available as the GRTC Core has added new, state-of-the-art equipment geared toward improving sensitivity. These upgrades include a nanospray source for the existing LC-Q mass spectrometer, a MALDI-TOF mass spectrometry, a high sensitivity fluorescence detector, and a new nanoprobe for the NMR. The availability of high quality instrumentation, coupled with the many combined years of analytical glycobiology experience represented by the members of the GRTC Glycotechnology Resource, allow us to meet the analytical challenges posed the Project Leaders and perform the analyses necessary to support their research aims.