4000 Q TRAP LC/MS/MS SHARED MASS SPECTROMETER: LIPIDOMICS

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请者提供)：弗吉尼亚联邦大学拥有一批非常活跃且不断增长的资助研究人员，他们致力于了解生物活性脂质的动态变化如何影响新陈代谢，以及调节正常和病理人类细胞功能的信号通路。需要为应用生物系统公司4000Q TRAP(Tm)LC/MS/MS系统、三重四极杆/线性离子陷阱质谱仪和一台液相色谱仪提供资金。这种异常灵活的仪器将最灵敏的三重四极杆质谱仪的独特功能与最高容量的离子陷阱结合在一起，提供组成脂体的各种分子的结构表征和定量所需的质量分辨率、灵敏度和动态响应。如本申请所述，来自7个不同科室的18名NIH资助的科学家和临床医生参与了超过45个NIH支持的研究项目，他们对这种质谱仪有特定的需求。4000Q陷阱将作为一个共享的研究资源运行，为我们正在进行的研究计划提供必要的支持，并促进新的协作研究计划，以鉴定、量化和表征脂质，特别强调细胞信号和功能脂质组学。提出这一请求的主要理由是，医学中心园区内没有具备所需功能的质谱仪。该仪器将在萨拉·斯皮格尔教授和威廉·M·格罗根教授的指导下作为VCU的核心资源进行管理，他们在脂类分析和核心资源的管理方面具有丰富的经验。理查德·莫兰教授将担任咨询委员会主席，监督该工具的公平使用，以造福于受资助的研究人员和大学研究环境。购买这种质谱仪对于继续开发和实现脂质组学研究的全部潜力至关重要，以更好地了解VCU医学中心园区的正常细胞功能和人类疾病。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Virginia Commonwealth University has a very active and growing critical mass of funded investigators working to understand how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for an Applied Biosystems 4000 Q TRAP(tm) LC/MS/MS System, a hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatograph. This exceptionally flexible instrument combines the unique capabilities of the most sensitive triple quadrupole mass spectrometer available with the highest capacity ion trap to provide mass resolution, sensitivity, and dynamic response needed for structural characterization and quantitation of the diverse molecules that comprise the lipidome. As described in this application, 18 NIH-funded scientists and clinicians from 7 different departments involved in over 45 NIH- supported research programs have specific needs for this mass spectrometer. The 4000 Q TRAP will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, and characterization, with particular emphasis on cell signaling and functional lipidomics. Providing a principal justification for this request, there is no mass spectrometer with the required capabilities on the Medical Center campus. The instrument will be administered as a VCU core resource under the direction of Professors Sarah Spiegel and William M. Grogan, who have extensive experience in lipid analysis and administration of core resources. Prof. Richard Moran will chair the Advisory Committee overseeing equitable use of the instrument for the benefit of funded investigators and the university research environment. The purchase of this mass spectrometer is essential to continuing development and realization of the full potential of lipidomics research toward better understanding of normal cellular function and human disease on the VCU Medical Center campus.