A Thermo Electron LTQ Mass Spectrometer

描述（由申请人提供）：将购买一台带有电喷雾和MALDI源的Thermo Electron Finnigan LTQ集成离子阱质谱仪、一台二维液相色谱仪和一台纳米电喷雾机器人，以满足波士顿大学医学院和波士顿大学戈德曼牙科医学院的一组主要用户开展的六个项目的需求，这两个项目都位于波士顿大学医学院。这些美国国立卫生研究院资助的研究人员从事(1)确定载脂蛋白B折叠的分子细节（Herscovitz）；(2) n -链糖基化在颌下腺发育中的作用（Kukuruzinska）；(3)硫酸肝素在血管平滑肌细胞增殖中的作用（Nugent）；(4)淀粉样疾病中转甲状腺素、免疫球蛋白轻链和血清淀粉样蛋白A的初级和翻译后修饰结构（Skinner）；(5) decorins在角膜生化中的作用（Trinkaus-Randall）；(6)结合生长因子（Zaia）的糖胺聚糖结构的测定。一组五名未成年使用者，四名来自波士顿大学医学院，一名来自纽约大学医学院，需要最先进的离子阱质谱法来支持牙周宿主-寄生虫相互作用的研究（Amar）；后翻译成骨桥蛋白（Berman）；秀丽隐杆线虫N-糖基化（Hirschberg）；小囊藻（Pilch）蛋白质和脂质成分的研究和分析n -糖基化的欧洲血小板（孙）。集成的LTQ离子阱质谱系统将被安置在BUSM共享质谱仪器实验室。将优先考虑所述的项目；在剩下的时间里，次要用户以及较大的BUSM社区将得到服务。

DESCRIPTION (provided by applicant): A Thermo Electron Finnigan LTQ integrated ion trap mass spectrometer with electrospray and MALDI sources, a two-dimensional liquid chromatograph, and a nano-electrospray robot will be purchased to meet the needs of six projects that are carried out by a group of major users based at Boston University (BU) School of Medicine and BU Goldman School of Dental Medicine, both located on the BU Medical Campus. These NIH-funded investigators are engaged in (1) determining the molecular details of the folding of apolipoprotein B (Herscovitz); (2) the role of N-linked glycosylation in submandibular gland development (Kukuruzinska); (3) the role of heparan sulfates in vascular smooth muscle cell proliferation (Nugent); (4) the primary and post-trranslational modification structures of transthyretin, immunoglobulin light chains, and serum amyloid A protein in amyloid diseases (Skinner); (5) the role of decorins in corneal biochemistry (Trinkaus-Randall); (6) determination of the structures of glycosaminoglycans that bind to growth factors (Zaia). A group of five minor users, four from the BU Medical Campus and one from New York University School of Medicine, have need for state-of-the-art ion trap mass spectrometry to support studies of periodontal host-parasite interactions (Amar); post-translations to osteopontin (Berman); C. elegans N- glycosylation (Hirschberg); studies of the protein and lipid components of caveolae (Pilch); and analysis of the N-glycosylation of uroplakins (Sun). The integrated LTQ ion trap mass spectrometry system will be housed in the BUSM Shared Mass Spectrometry Instrumentation Laboratory. Priority will be given to the projects described; minor users as well as the larger BUSM community will be served in the remaining time. The instrument will be operated and maintained by experienced personnel. The costs of instrument operation, supplies, and service contract will be shared by the users, as stated in their support letters. The salary for the instrument operator will be provided by the Dean of BU School of Medicine.