MICROARRAY SCANNER AND ACCESSORIES FOR MUSC

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：通过使用DNA微阵列技术进行基因表达的高通量分析已经成为生物医学研究的有力工具。它的应用范围很广，从单核苷酸多态性的测序和表征，到生物或致病表型的诊断，以及生物样品的转录组学分析。南卡罗来纳州医科大学（MUSC）DNA微阵列和生物信息学设施成立于2001年，为来自MUSC和其他南卡罗来纳州机构的研究人员提供全面的基础设施，包括教师，支持人员，最先进的仪器和先进的计算/统计分析资源，以进行DNA微阵列分析。迄今为止，该设施已在30多个研究项目中实施，包括南卡罗来纳州的18名NIH资助的研究人员。然而，微阵列技术的新进展，更高水平的通量筛选的需求，以及我们目前的微阵列仪器即将过时，需要新的仪器的收购，以促进正在进行的和未来的微阵列实验在MUSC。该提案将概述微阵列技术目前对MUSC研究人员的重要性，MUSC现有微阵列仪器的局限性，以及更换这种仪器和增加额外技术能力的必要性。具体而言，请求提供资金用于购买Affyestry GeneChip扫描仪3000升级版和Fluidics Station 450升级版。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): High throughput analysis of gene expression through the use of DNA microarray technology has become established as a powerful tool for biomedical research. Its applications are broad, ranging from sequencing and characterization of single nucleotide polymorphisms, to diagnosis of biological or pathogenic phenotypes, and to the transcriptomic profiling of biological samples. The Medical University of South Carolina (MUSC) DNA Microarray and Bioinformatics Facility was established in 2001 to provide investigators from MUSC and other South Carolina institutions with a comprehensive infrastructure comprised of faculty, support staff, state-of-the-art instrumentation and advanced computational/statistical analysis resources required to perform DNA microarray analysis. To date, this facility has been implemented in over 30 research projects, including those of 18 NIH-funded investigators in South Carolina. However, new advances in microarray technology, the demand for even higher levels of throughput screening, and the imminent obsolescence of our present microarray instrumentation necessitate the acquisition of new instrumentation in order to facilitate ongoing and future microarray experimentation at MUSC. This proposal will outline the current importance of microarray technology to MUSC investigators, the limitations of existing microarray instrumentation at MUSC, and the necessity for replacing this instrumentation as well as adding additional technical capabilities. Specifically, funds are requested for purchasing an Affymetrix GeneChip Scanner 3000 Upgrade and a Fluidics Station 450 Upgrade.