Bone Lead Standardization Program

描述（由申请人提供）：本提案旨在创建通过参考方法和体内X射线荧光（XRF）获得的骨铅测量（BLSP）的标准化程序。BLSP将满足骨电极导线方法学的当前需求：缺乏适当的经认证参考物质（RM）和缺乏可追溯性。BLSP还将为原子吸收光谱法（AAS）和电感耦合等离子体质谱法（ICP-MS）提供RM，并将在使用这些方法的实验室之间进行实验室间验证研究。该计划还将为XRF提供RM，从而提供SI单位的可追溯性，并将在使用XRF进行体内骨铅测量的实验室之间进行实验室间验证研究。有六个目标：第一，在纽约州卫生部（NYS DOH）的沃兹沃斯微量元素实验室交叉验证AAS和ICP-MS;第二，通过可追溯到NIST SRM 1486骨粉和1400骨灰的AAS和ICP-MS建立骨铅测量的RM的国家储存库;第三，进行骨铅测量的AAS和/或ICP-MS的实验室间验证。第四个目标是通过制备两组表征良好的山羊骨样本，组装一个用于骨铅XRF的RM的国家储存库，所述山羊骨样本含有包含在人群中发现的内源性铅浓度;第五个目标是使用两组山羊骨样本进行体外XRF实验室间验证和比较研究。作为通过AAS和ICP-MS获得可溯源至SI单位的表面骨和核心骨参考浓度过程的一部分，将销毁第一组XRF RM。由此获得的参考浓度将构成XRF准确度评估的基础。将创建第二组XRF RM，用于第二次实验室间比较，并将长期保存。第六个也是最后一个目标是使用将提供RM的NYS DOH山羊群的安乐死成员进行体内XRF确认研究。将XRF测量的体内浓度与AAS和ICP-MS获得的参比浓度进行比较。

DESCRIPTION (provided by applicant): The aim of this proposal is to create a Standardization Program for Bone Lead measurements (BLSP) obtained via reference methods and via in vivo X-Ray Fluorescence (XRF). The BLSP will fulfill current needs of the bone lead methodologies: lack of appropriate certified reference materials (RMs) and lack of traceability. The BLSP will also provide RMs for electrothermal atomic absorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS) and will conduct an interlaboratory validation study among laboratories using those methods. The program will also provide RMs for XRF, thereby providing traceability to SI units, and will conduct an interlaboratory validation study among laboratories using XRF for in vivo bone lead measurements. There are six goals: first to cross-validate AAS and ICP-MS in the Wadsworth Trace Elements Laboratory of the New York State Department of Health (NYS DOH); second to establish a national repository of RMs for bone lead measurement via AAS and ICP-MS traceable to NIST SRM 1486 Bone Meal and 1400 Bone Ash; third, to conduct an interlaboratory validation of AAS and/or ICP-MS for bone lead measurement. The fourth goal is to assemble a national repository of RMs for bone lead XRF by preparing two sets of well-characterized goat bone samples containing endogenous lead concentrations that encompass those found in human populations; the fifth goal is to conduct an in vitro XRF interlaboratory validation and comparison study using the two sets of goat bone samples. The first set of XRF RMs will be destroyed as part of the process of obtaining reference concentrations via AAS and ICP-MS for both surface and core bone that are traceable to SI units. The reference concentrations thus obtained will form the basis of the assessment of XRF accuracy. The second set of XRF RMs will be created and used for a second interlaboratory comparison and will be preserved for long-term use. The sixth and last goal is to conduct an in vivo XRF validation study using the euthanized members of the NYS DOH goat colony that will provide the RMs. XRF-measured in vivo concentrations will be compared to the reference concentrations obtained from AAS and ICP-MS.