Surgery, Physiology and Immunohistopathology

外科、生理学和免疫组织病理学核心(核心C)，将由所有 该PPG的项目将提供综合服务，以评估心脏功能和结构在以下环境中 在小鼠身上模拟心脏病理。因此，核心C将安置和维护设备，并提供 为项目负责人和其他PPG人员提供以下服务所需的工作人员： 1)手术：活体心肌梗死和经主动脉缩窄， 2)生理学：超声心动图、朗宁多夫和工作的小鼠心脏， 3)免疫组织病理学：最先进的心脏和心肌细胞共聚焦荧光显微成像 小鼠心脏切片和培养细胞中信号分子的结构和细胞位置。 生理学、外科和免疫组织病理学核心，核心C，将集中在最近 已建成的SDSL)生物科学中心，是SDSU心脏研究所实验室和行政办公室的所在地。 核心C由设备齐全的手术和生理套房、专门的老鼠休息室、 完整的组织学实验室和最先进的共聚焦显微镜成像设备。PPG调查员 在所有三个机构，以及细胞生物学核心的活细胞成像中心，将能够连接 通过开放显微镜环境(OME)连接到Core C成像设备。OME将优化形象 所有5个PPG项目的数据分析、传输和存储，以及促进固定项目的整合 C芯的样品成像和B芯的活细胞成像。C芯的另一个优势是它 毗邻圣地亚哥州立大学心脏研究所老鼠遗传中心，该中心促进了 所有PPG项目都对小鼠进行了转基因。Core C专门为这种多机构服务而设计 PPG；因此，它将最大化项目之间的协作，从而增强协同效应 这一综合研究项目。核心C与核心B相辅相成，通过提供理想的 在微观层面上研究细胞结构的场所，并作为 使用细胞生物学核心中产生的细胞发现的原理可以在病理生理学中进行检查 模拟体内环境的环境。

The Surgery, Physiology and Immunohistopathology Core (Core C), which will be used by all of the Projects of this PPG, will offer integrated services for assessing heart function and structure in settings that mimic cardiac pathology in mice. Accordingly, Core C will house and maintain the equipment and provide the staff necessary to offer Project Leaders and other PPG personnel the following services: 1) Surgery: in vivo myocardial infarction and transaortic constriction, 2) Physiology: echocardiography, Langendorff and working mouse hearts, 3) Immunohistopathology: state-of-the-art confocal fluorescent microscopic imaging of heart and myocyte structure, and cellular locations of signaling molecules in mouse heart sections and cultured cells. The Physiology, Surgery and Immunohistopathology Core, Core C, will be centrally located in the recently completed SDSL) BioScience Center, which houses the SDSU Heart Institute labs and administrative offices. Core C is composed of fully equipped surgery and physiology suites, dedicated mouse holding rooms, a complete tissue histology lab and a state-of-the-art confocal microscopy imaging facility. PPG investigators at all three institutions, as well as the live-cell imaging center in the Cell Biology Core, will be able to connect to the Core C imaging facility via an open microscopy environment (OME). The OME will optimize image data analysis, transfer and storage for all 5 of the PPG projects, as well as facilitating the integration of fixed sample imaging in Core C with live-cell imaging in Core B. An additional advantage of Core C is that it adjoins the SDSU Heart Institute Mouse Genetics Center, which facilitates the generation and use of genetically modified mice by all of the PPG projects. Core C was designed specifically to serve this multiinstitutional PPG; accordingly, it will maximize collaborations between projects, thus enhancing the synergy of this integrated research program. Core C complements and integrates with Core B by providing the ideal venue for examining cellular structure at the microscopic level, and by serving as the location where principles discovered using cells generated in the Cell Biology Core can be examined in a pathophysiological context that mimics the in vivo setting.