Gamma Irradiator

描述(由申请人提供)：本申请的主要目标是购买一台Gammacell 40 Expertor，一种伽马射线照射器，用于照射小鼠、细胞和组织。该仪器的主要用户是美国国立卫生研究院资助的研究人员，他们的工作严重依赖于伽马辐照装置的使用。该应用程序中的主要用户项目涵盖多个学科，包括：小鼠和人源化小鼠的内皮祖细胞功能和血管生成；小鼠的造血干细胞移植、分化和白血病发展；研究铁稳态调节的小鼠模型；小鼠造血细胞发育中的氧化应激；造血干细胞抗HIV-1基因传递和HIV-1致病研究的人源化小鼠模型；细胞移植和异种胰岛移植的小鼠模型；自身免疫病模型；乳腺癌转移；临床前研究，使用小鼠模型评估活化蛋白C降低由DNA损伤诱导的细胞周期检查点控制引起的死亡率的能力；以及小干扰RNA和核酶在治疗艾滋病毒感染中的作用。我们要求最先进的伽马辐照器，使我们能够继续目前资助的研究，并将满足预期增加的辐照器接入需求，由于容量限制和高龄导致的机械功能退化，我们目前已有27年历史的143-35型伽马辐照器无法满足这些需求。 目的：这项建议的长期目标是为分子和实验医学系和斯克里普斯研究所的研究人员提供一种新一代伽马辐照装置，为小动物的治疗提供人道、可靠、可重复性和无故障的照射。该仪器还将用于细胞和组织的治疗。 相关性：确定的用户群体都参与了与人类健康高度相关的疾病特定研究，并得到了几个NIH研究所的支持。研究范围从对基本细胞和分子机制的洞察到临床前模型。项目说明：

DESCRIPTION (provided by applicant): The major goal of this application is to purchase a Gammacell 40 Exactor, a gamma irradiator, for use in irradiation of mice, cells, and tissue. The major users of the instrument are NIH funded investigators whose work is heavily dependent on the use of a gamma irradiator device. Major users projects included in this application cover a variety of disciplines, including: endothelial progenitor function and angiogenesis in mice and humanized mice; hematopoietic stem cell transplantation, differentiation, and leukemia development in mice; mouse models for investigating the regulation of iron homeostasis; oxidant stress in hematopoietic cell development in mice; humanized mouse models for hematopoietic stem cell anti-HIV-1 gene delivery and HIV-1 pathogenic studies; mouse models for cell transplantation and islet xenotransplantation; autoimmune disease models; breast cancer metastasis; preclinical studies using mouse models to evaluate the ability of activated protein C to reduce mortality caused by radiation DNA damage induced cell cycle check point control; and small interference RNA and ribozymes in treating HIV infection. We are requesting a state-of-the-art gamma irradiator that will allow us to continue currently funded studies and will accommodate anticipated increased demand for irradiator access that cannot be met by our current 27 year old Model 143-35 gamma irradiator due to limitations of capacity and degrading mechanical function due to advanced age. Objective: The long-term objective of this proposal is to provide researchers at The Department of Molecular and Experimental Medicine and The Scripps Research Institute a current generation gamma irradiator that provides humane, reliable, reproducible, and trouble free irradiation for the treatment of small animals. This instrument will also be used for treatment of cells and tissues. Relevance: Identified user groups are all involved in disease specific research that is highly relevant to human health, and receive support from several NIH institutes. The investigations span the research spectrum from insight into basic cellular and molecular mechanism to preclinical models.7. Project narrative: