Core B - Animal Exposure and Assessment

动物暴露和评估核心的总体目标是为研究人员提供支持， 氧化性空气污染物对幼年恒河猴过敏性气道疾病的影响。核心被安置在 加州国家灵长类动物研究中心（CNPRC）的暴露设施。我们的项目团队 最近建立了一种非人灵长类动物过敏性气道疾病模型， 婴儿过敏性气道疾病我们的模型是在恒河猴中使用已知的人类过敏原， 屋尘螨。我们已经发现，臭氧（O3）共同暴露显着 会加重病情CNPRC的风险敞口机制拥有完善的机制，可评估所有 气道反应性、免疫应答以及暴露于过敏原和氧化剂气体方面， 暴露条件，并有一个完整的肺功能测试单元。具体而言，该核心将： 1.提供准确和仔细控制的动物或体外制剂暴露于选定的 空气污染物的种类和浓度。 2.制定吸入暴露和大气特征的方法。 3.协调实验动物的购买、卫生服务和护理。 4.提供后勤和技术支持，以确保正确实施实验方案。 5.制备HDM过敏原，用于恒河猴幼猴致敏和攻毒。 6.使用我们小组开发的已发表的方案使婴儿恒河猴对HDM过敏原致敏。 7.使用皮肤点刺试验验证对HDM过敏原的致敏性。 8.为研究人员提供患有仔细定义的过敏性气道疾病的恒河猴幼崽， 仔细控制和验证氧化剂气体和HDM过敏原的暴露条件。 9.提供详细记录的气道反应性和肺力学和容量的状态， 幼年恒河猴 10.整理所有暴露、致敏和肺功能数据，并将其提供给实验 单位 11.在尸检时协调组织采集，并保存采集档案和样本 保管和存储日志。

The overall goal of the Animal Exposure and Assessment Core is to provide support to investigators studying the impact of oxidant air pollutants on allergic airways disease in infant rhesus monkeys. The Core is housed in the Exposure Facility at the California National Primate Research Center (CNPRC). Our Program Project team has recently established a non-human primate model of allergic airways disease that closely matches human infant allergic airways disease. Our model is generated in rhesus monkeys using a known human allergen, the house dust mite (HDM; Dermatophagoides famiae). We have found that ozone (O3) co-exposure markedly enhances the disease. The Exposure Facility at the CNPRC has well-established facilities for assessing all aspects of airways reactivity, immune response, and exposure to allergens and oxidant gases with validated exposure conditions, and it has a complete pulmonary function testing unit. Specifically, this Core will: 1. Provide accurate and carefully controlled exposures of animals or in vitro preparations to selected types and concentrations of air pollutants. 2. Develop methods of inhalation exposure and atmosphere characterization. 3. Coordinate the purchase, health services, and care of experimental animals. 4. Provide logistical and technical support to ensure the correct implementation of experimental regimens. 5. Prepare HDM allergen for use in sensitization and challenge of infant rhesus monkeys. 6. Sensitize infant rhesus monkeys to HDM allergen using a published protocol developed by our group. 7. Verify sensitization to HDM allergen using skin prick tests. 8. Provide researchers with infant rhesus monkeys with carefully defined allergic airways disease using carefully controlled and validated exposure conditions for oxidant gases and HDM allergen. 9. Provide carefully documented status of airway responsiveness and lung mechanics and volumes in infant rhesus monkeys. 10. Collate all exposure, sensitization, and pulmonary function data and provide this to the experimental units. 11. Coordinate the acquisition of tissues at necropsy and maintain the collection archive and sample custody and storage log.