Functional Imaging of Freely Moving Animals

描述（由申请人提供）：我们建议开发一种双模式光学EEG传感系统，该系统可在自由移动的动物中提供4D功能成像。所提出的系统采用了拴系的安排，连接到一个两阶段的可拆卸的测量头与一端手术固定到大鼠的头部。这允许同时收集全脑时间序列DOT和多导联电生理数据，同时允许动物参与复杂的行为。计划是一个系统的计算，实验室和动物研究，将有助于定义在现实条件下的性能和数据质量的保真度的限制。获得的初步数据表明，全头血流动力学响应门控行为依赖性海马激活状态是空间上不同的和可重复的。拟议的系统提供了一个独特的机会，探索依赖范围广泛的复杂行为的基础神经和神经血管耦合事件，并应证明具有重要价值的基础神经科学和制药界。

DESCRIPTION (provided by applicant): We propose to develop a dual-mode, optical-EEG sensing system that provides for 4D functional imaging in freely moving animals. The proposed system employs a tethered arrangement that attaches to a two-stage detachable measuring head with one end surgically fixed to the head of a rat. This allows for concurrent collection of whole brain time-series DOT and multilead electrophysiological data while permitting the animal to engage in complex behaviors. Planned are a systematic computational, laboratory and animal studies that will serve to define the limits of performance and fidelity of data quality under realistic conditions. Preliminary data obtained shows that whole head hemodynamic responses gated to behavior dependent hippocampal activation states are spatially distinct and reproducible. The proposed system offers a unique opportunity to explore the dependence of a wide range of complex behaviors on underlying neural and neuro-vascular coupling events and should prove of significant value to the basic neuroscience and pharmaceutical communities.