Immunotoxicology Core

免疫毒理学核心将作为一个中央设施，协助中心的调查人员 对这三个项目进行高质量的研究。此外，它还将作为毒性测试的核心 所有项目通过筛选本申请中使用的各种植物衍生化合物的系统毒性 以及免疫毒性。这是必要的，因为尽管CAM被认为是无毒的，但草药 在柜台上出售的提取物或提纯成分可能会产生有毒影响。此外，虽然 项目涉及的研究主要限于T细胞，了解这种植物是如何衍生出来的是至关重要的 这些成分会影响整个免疫系统。这一点尤其重要，因为自体免疫和 炎症性疾病通常是全身性的，涉及多种类型的免疫细胞。此外， 有关植物衍生物的免疫毒性作用的信息在开发床边床上是至关重要的 研究。 免疫毒理学核心的具体目标是 1)检测被调查植物产品的免疫毒性作用 2)提供共享资源和技术援助，以从事前沿研究 个别项目 3)向该中心的调查人员提供使用主要设备的培训 4)协助及时收集和分析数据 5)实现项目之间的数据共享，从而加强协作 6)参与研究项目相关实验的开发和实施。 这个核心有两个主要组成部分。他们是 1)免疫毒性测试，包括a)标准毒性测试b)一级免疫毒性测试c) 二级免疫毒性测试。免疫毒性测试将包括急性、短期和长期研究。 白藜芦醇、葡萄籽/葡萄皮提取物用于项目1、西洋参及其活性成分用于项目 2和项目3的大麻酚(CBD)和富含CBD的馏分。 2)仪器资源基金，它将a)提供共享资源，以提高研究质量 在该中心进行，b)使调查人员能够获得、分析和分享3个月的数据 项目。这将包括由一名生物统计学家提供的统计支助，c)培训 使用核心设施。 总之，免疫毒理学核心将提供有效和经济的共享资源 开展前沿研究，整合该中心的所有项目。

The Immunotoxicology Core will serve as a centralized facility to assist the investigators at the Center to pursue high quality research on the three projects. In addition, it will also serve as a toxicity testing core for all projects by screening the various plant-derived compounds used in this application for systemic toxicity as well as immunotoxicity. This is necessary because although CAM is considered to be non-toxic, the herbal extracts or purified ingredients that are sold over the counter may exert toxic effects. In addition, while the projects deal with studies restricted primarily to T cells, it is critical to know how such plant-derived components affect the overall immune system. This is especially important because autoimmune and inflammatory diseases are often systemic and involve multiple types of immune cells. Furthermore, information on the immunotoxic effects of the plant derivates is critical in the development of bench-tobedside research. The specific aims of the Immunotoxicology Core are 1) To examine the immunotoxic effects of the plant products under investigation 2) To provide the shared resources and technological assistance to pursue cutting-edge research on the individual projects 3) To provide training to investigators at the Center in the use of major equipment 4) To aid data collection and analysis in a timely manner 5) To enable data sharing between the projects, thereby enhancing collaborations 6) To participate in the development and conduct of experiments related to the research projects. There are two main components of this core. They are 1) Immunotoxicity testing which will include a) Standard Toxicity Testing b) Tier 1 Immunotoxicity Testing c) Tier 2 Immunotoxicity Testing. The Immunotoxicity testing will involve acute, short-term and chronic studies of resveratrol, grape seed/skin extracts for Project 1, American ginseng and its active ingredient for Project 2, and cannabidol (CBD) and CBD-rich fractions for Project 3. 2) Instrumentation Resources Facility which will a) Provide shared resources to enhance quality of research conducted at the Center, b) Enable data acquisition, analysis and sharing between investigators on the 3 projects. This would include statistical support provided by a biostatistician, c) Training of personnel in the use of core facilities. In summary, the Immunotoxicology Core will provide effective and economical shared resources to conduct cutting-edge research that leads to integration of all the projects at the Center.