Vibrio cholerae Biofilm Development

霍乱是一种严重的流行性腹泻疾病，由革兰氏阴性水生细菌弧菌引起。 霍乱弧菌。在一种称为生物膜的多层结构中附着在生物表面上对 霍乱弧菌等细菌病原体的存活和毒力。生物膜的形成被认为是一种 发展过程。在这个过程的第一阶段，细菌在表面形成一个单层。 单层的形成依赖于细胞-表面的相互作用。当启用细胞-细胞交互时， 单层生物膜发育成多层生物膜。 在这笔赠款的前一个资助期内，我们专注于定义环境信号， 霍乱弧菌单层和生物膜的遗传要求和基因转录图谱。具体的 这项提案的目标源于前一个资助期内的三项发现，具体如下 以下是： 1)研究富含葡萄糖环境中表面相关生长的调节，并确定 它与宿主胃肠道内的生存相关。 观察：葡萄糖特异性调节生物膜相关细胞进入静止期 成长。 2)鉴定霍乱弧菌生物膜基质的蛋白质组分。 观察：霍乱弧菌生物膜的形成需要两种假定的凝集素。 3)研究十一碳烯基焦磷酸酶在霍乱弧菌生物膜基质中的作用 综合。 观察：生物膜特别需要一种假定的十一碳烯基焦磷酸酶 形成，但不是细胞生长。

Cholera is a severe epidemic diarrheal disease caused by the gram-negative aquatic bacterium Vibrio cholerae. Attachment to biotic surfaces in a multi-layered structure known as a biofilm is critical to the survival and virulence of bacterial pathogens such as V. cholerae. Biofilm formation is viewed as a developmental process. In the first stage of this process, the bacterium forms a monolayer on the surface. Formation of a monolayer depends on cell-surface interactions. When cell-cell interactions are enabled, the monolayer develops into a multi-layered biofilm. During the previous funding period of this grant, we focused on defining the environmental signals, genetic requirements, and gene transcription profiles of the V. cholerae monolayer and biofilm. The Specific Aims of this proposal arise from three discoveries made during the previous funding period and are as follows: 1) To investigate regulation of surface-associated growth in glucose rich environments and to define its relevance to survival within the host gastrointestinal tract. Observation: Glucose specifically regulates the entry of biofilm-associated cells into the stationary phase of growth. 2) To characterize the proteinaceous components of the Vibrio cholerae biofilm matrix. Observation: Two putative lectins are required for V. cholerae biofilm formation. 3) To study the role of an undecaprenyl pyrophosphate phosphatase in V. cholerae biofilm matrix synthesis. Observation: A putative undecaprenyl pyrophosphate phosphatase is specifically required for biofilm formation but not cell growth.