Quadrupole Ion Trap/FTICR Tandem Mass Spectrometer

描述(申请人提供)：本建议的目的是开发一种新的串联质谱仪(MS/MS)技术，用于生物分子的质量和结构分析，该技术可以结合四极离子陷阱(QIT)的出色离子隔离和碎裂能力与傅立叶变换(FT)离子回旋共振(ICR)质谱仪(MS)的高分辨率和高质量精度。这项技术的主要特点是在所建议的仪器的ICR部分使用永磁体来产生磁场，这将使其具有成本效益、体积紧凑以及对许多应用来说负担得起的特点。主要的重点是在最大空间体积中产生均匀的磁场，该磁场在一定程度上可以补偿由ICR单元中的永久磁体产生的较低磁场(约1-1.5特斯拉，而超导磁体产生的磁场为7-12特斯拉)的使用造成的限制。为了实现这一目标，提出了一种新的永磁体设计方案。预计新仪器将具有商用QIT-MS系统的尺寸、成本和MS/MS能力，分辨率和质量精度将超过流行的混合四极/飞行时间(QqTOF)MS。

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a novel tandem mass spectrometry (or MS/MS) technology for mass and structural analysis of biomolecules that could combine excellent ion isolation and fragmentation capabilities of a quadrupole ion trap (QIT) and high resolution and mass accuracy of Fourier transform (FT) ion cyclotron resonance (ICR) mass spectrometry (MS). The main feature of this technology is the use of a permanent magnet for generation of magnetic field in ICR part of the proposed instrument that will make it cost-efficient, compact in size, and affordable for many applications. The major emphasis is made on producing a homogeneous magnetic field in a maximum space volume that to a certain extent can compensate limitations imposed by the use of a lower magnetic field generated by a permanent magnet in the ICR cell (about 1-1.5 Tesla compared to 7-12 Tesla generated by superconductive magnets). A new permanent magnet design is suggested to achieve this goal. The new instrument is expected to have size, cost and MS/MS capabilities of commercial QIT-MS systems and the resolving power and mass accuracy exceeding that of a popular hybrid quadrupole/time-of-flight (QqTOF) MS.