Development of Peromyscus Genomics

描述（由申请人提供）：Peromyscines是分布最广泛和最丰富的北美本土哺乳动物。最近，白足鹿鼠（Peromyscus leucopus）和马尼拉鹿鼠（P maniculatus）突然引起了全国的关注，因为它们被发现是引起两种新出现的传染病（莱姆病和汉坦病毒肺综合征）的主要微生物宿主。被恰当地称为“北美哺乳动物的果蝇”的peromyscines也被认为是研究1）负责生殖隔离和物种形成的基因，和2）使生理和行为适应于变化的环境条件、适应于其他物种、相互适应以及适应于微生物和其他寄生虫的基因的理想模型。 利用这种潜力的一个重要工具是连锁图。这是本建议的目的是开发一个中等密度的连锁图谱的P maniculatus bairdii使用PCR标记在足够的密度，约5-10 cM，允许识别的主要部分同线的参考物种，小家鼠。这些标记包括1）对同线性鉴定重要的I型（蛋白质编码基因），和2）高度多态性并最终将用于QTL分析的II型（微卫星）。连锁分析面板被选择为最大限度地利用多态性，是由种间减数分裂Segregants的姐妹种P m bairdii和P polionotus之间的杂交。 Peromyscus连锁图谱的开发将显着提高物种的研究潜力，加强目前正在进行的项目，并鼓励其他项目的开发，探索宿主-寄生虫相互作用，物种形成，行为和适应性性状获得的各个方面的遗传学。通过这样的图谱实现的特定信息包括QTL分析、遗传操作和单个QTL的解剖，以及单个QTL的潜在候选基因的鉴定。

DESCRIPTION (provided by applicant): Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and Hantaviral pulmonary syndrome. Aptly called "The Drosophila of North American Mammology" peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, approximately 5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. The linkage analysis panel was selected for maximizing utilizable polymorphism and is composed of Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus. Development of a Peromyscus linkage map will markedly improve the research potential of the species, enhancing projects currently underway and encouraging the development of other projects that explore the genetics of host-parasite interactions, speciation, behavior and the various aspects of adaptive trait acquisition. Specific information enabled by such a map includes QTL analysis, genetic manipulation and dissection of individual QTLs, and identification of potential candidate genes for individual QTLs.