Peromyscus Laboratory Models for Biomedical Research

描述(由申请人提供)：Permyscus Genetic Stock Center(PGSC)提供了可靠的无病Permyscus来源，并且是可用的Permyscus物种和突变体的无与伦比的。PGSC为各种领域提供动物，包括人类传染病、行为研究、生理适应和遗传学研究。两项进展应该会进一步提高人们对Permyscus作为实验生物体的兴趣。根据我们提交的白皮书，NHGRI选择了一个物种的6X序列和其他3个物种的2X序列。这个项目在贝勒医学院刚刚开始。在与Glenn博士(美国佐治亚州)的合作下，向JGI/DOE提出了50,000个Permyscus EST克隆的构建和测序请求。已经进行了EST克隆，并完成了质量评估。这些完整的序列数据应该在大约一个月内可用。此外，第一代连锁图谱显示，Permysus和Rattus之间的连锁关系比Permyscus和Mus之间的连锁关系更近。进一步的连锁和染色体绘制分析使得构建基于染色体的连锁图谱成为可能。这些资源将加强Permyscus作为研究工具在重复运动障碍、青少年和成人共济失调、癫痫、疾病中的基因表达和环境毒理学以及基因组印迹机制等领域的研究工具的重要性。Permyscus是两种新兴感染微生物的储藏库。汉塔病毒导致的疾病死亡率为40%，是一种潜在的生物恐怖主义因子。莱姆病的病原体也藏匿在Permyscus中，每年发生近15,000例。PGSC为研究这些疾病和其他疾病，如埃立克斯虫病、巴贝斯虫病和斑块的研究人员提供了动物。该项目的研究方向是继续开发胚胎冷冻保存方法和活动物的再衍生，以保存稀有突变和种质，以备将来需要时使用，并降低成本。在通过修改小鼠使用的激素治疗方案来增加胚胎产量方面取得了进展。胚胎已经成功冷冻和解冻，可以进行细胞分裂。未来的工作将进一步改进这些方法，目标是从冷冻胚胎中重新衍生出活的动物。

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos.