Peromyscus Laboratory Models for Biomedical Research

描述（由申请人提供）：Peromyscus遗传资源中心（PGSC）提供无病Peromyscus的可靠来源，并且对于可用的Peromyscus物种和突变体的多样性是无与伦比的。PGSC为各种领域提供动物，包括人类传染病，行为研究，生理适应和遗传研究。两项研究进展将进一步提高人们对石原菌作为实验生物的兴趣。根据我们提交的白皮书，NHGRI选择对一个物种进行6X测序，对另外3个物种进行2X测序。这个项目正在贝勒医学院进行。与Dr. Glenn （U. Georgia）合作，向JGI/DOE提出了5万个Peromyscus EST克隆的构建和测序请求。EST克隆已经完成，质量评估也已经完成。完整的序列数据应在大约一个月内提供。此外，第一代连锁图谱显示Peromysus与Rattus的亲缘关系比Peromyscus与Mus的亲缘关系更密切。进一步的连锁和染色体绘制分析使得基于染色体的连锁图谱得以构建。这些资源将增强Peromyscus作为重复运动障碍、青少年和成人共济失调、癫痫、疾病和环境毒理学基因表达以及基因组印记机制等领域基因发现研究工具的重要性。Peromyscus是两种新兴感染性微生物的储藏库。汉塔病毒导致的疾病死亡率高达40%，是潜在的生物恐怖主义制剂。莱姆病的病原体也窝藏在Peromyscus中，每年发生近15,000例。PGSC为研究这些疾病和其他疾病如埃利希体病、巴贝斯虫病和斑块的研究人员提供了动物。该项目的研究方向是继续发展胚胎冷冻保存方法和活体动物的再衍生，以保存稀有突变体和种质，以备将来需要时使用，并降低成本。通过对小鼠的激素治疗方案进行修改，在增加胚胎产量方面取得了进展。胚胎已经成功地冷冻和解冻，以进行细胞分裂。未来的工作将进一步完善这些方法，目标是从冷冻胚胎中重新衍生出活体动物。

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos.