Peromyscus Laboratory Models for Biomedical Research

用于生物医学研究的 Peromyscus 实验室模型

• 批准号: 8464295
• 负责人: Michael Ray Felder
• 金额: $ 23.28万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8464295
• 关键词: Adolescent; Adult; American; Animal Experimentation; Animal Model; Animals; Area; Ataxia; Babesiosis; Basic Science; Behavioral; Biomedical Research; Bioterrorism; Breeding; Cell division; Chromosome Mapping; Chromosome Painting; Chromosomes; Collaborations; Communicable Diseases; Communities; Cryopreservation; Data; Deer Mouse; Derivation procedure; Detection; Development; Disease; EST Library; Ehrlichiosis; Embryo; Embryo Transfer; Embryonic Development; Environment; Epidemiology; Epilepsy; Expressed Sequence Tags; Freezing; Future; Gene Expression; Generations; Genetic; Genetic Variation; Genome; Genomic Imprinting; Genomics; Goals; Grant; Habitats; Hormonal; Human; Hybrids; Individual; Institutes; Internet; Investigation; Joints; Laboratories; Laboratory Organism; Large-Scale Sequencing; Letters; Life; Lyme Disease; Methods; Microarray Analysis; Modeling; Modification; Movement Disorders; Mus; National Human Genome Research Institute; Paper; Peromyscus; Physiological; Physiological Adaptation; Physiology; Principal Investigator; Production; Publications; Rattus; Research; Research Personnel; Resources; Rodent; Single Nucleotide Polymorphism; Social Behavior; Source; Study models; Syndrome; Technology; Universities; Virus; Work; Xenobiotic Metabolism; base; cost; environmental toxicology; gene discovery; genetic linkage; genetic manipulation; interest; medical schools; microorganism; mortality; mutant; programs; public health relevance; tool

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos.

描述（由申请人提供）：Peromyscus 遗传库存中心 (PGSC) 提供了无病 Peromyscine 的可靠来源，并且在 Peromyscus 物种和突变体的多样性方面是无与伦比的。 PGSC 为各个领域提供动物，包括人类传染病、行为研究、生理适应和遗传研究。两项进展应该会进一步增强人们对白鼠属作为实验生物的兴趣。根据我们提交的白皮书，NHGRI 选择对一个物种进行 6X 测序，对其他 3 个物种进行 2X 测序。该项目刚刚在贝勒医学院进行。与 Glenn 博士（美国佐治亚州）合作，向 JGI/DOE 请求构建 50,000 个 Peromyscus EST 克隆并进行测序。 EST 克隆已经完成，质量评估也已完成。这一完整的序列数据应在大约一个月内提供。此外，第一代连锁图谱揭示了白鼠属和鼠属之间的连锁关系比白鼠属和鼠属之间的连锁关系更紧密。进一步的连锁和染色体绘制分析允许构建基于染色体的连锁图谱。这些资源将增强 Peromyscus 作为研究工具在重复性运动障碍、青少年和成人共济失调、癫痫、疾病和环境毒理学中的基因表达以及基因组印记机制等领域的基因发现领域的重要性，Peromyscus 是两种新兴传染性微生物的储存库。汉塔病毒引起的疾病死亡率为 40%，是潜在的生物恐怖主义媒介。莱姆病的病原体也藏匿在白鼠属中，每年发生近 15,000 例病例。 PGSC 为研究这些疾病以及埃利希体病、巴贝斯虫病和斑块等其他疾病的研究人员提供了动物。该项目的研究方面是继续开发胚胎冷冻保存方法和活体动物再衍生，以保存稀有突变体和种质以供将来需要时使用并降低成本。通过修改小鼠的激素治疗方案，在增加胚胎产量方面取得了进展。胚胎已成功冷冻和解冻以进行细胞分裂。未来的工作将进一步完善这些方法，目标是从冷冻胚胎中重新衍生出活体动物。