RNAi for the identification of Hypoxia responsive genes

最近启动了一个项目，利用RNAi对缺氧反应基因进行系统分析。一些研究人员对研究细胞的缺氧反应很感兴趣。通过引入shRNA，可以将缺氧的生物刺激引入HeLa、7860肾细胞CA或DU-145(有或没有药物干预)细胞系中，作为一种解剖缺氧途径的手段。到目前为止，我们已经分析了针对以下缺氧相关基因-HIF-1A、HIF1AN-1和HIF3A的siRNAs。后续验证和表型分析已经开始。此外，我们还通过Open BiosSystems购买了对应于HIF1a、HIF2A、ARNT、HSPCA和VEGF的shRNA克隆。在RNA水平验证之后，我们将能够评估来自CSHL的shRNA克隆的工作效率，并将能够将其用于小规模功能分析。

A project has been recently initiated to conduct a systematic analysis of hypoxia responsive genes using RNAi. Several investigators are interested in examining the hypoxic response of cells. The biologic stimulus of anoxia could be introduced into cell lines including HeLa, 7860 Renal Cell CA or DU-145 (with or without drug intervention) following shRNA introduction as a means of dissecting components of the hypoxia pathway. To date we have analyzed siRNAs against the following hypoxia associated genes -HIF-1A, HIF1An-1 and HIF3A. Follow up validation and phenotypic analysis has begun. In addition, we have purchased through Open Biosystems shRNA clones corresponding to HIF1A, HIF2A, ARNT, HSPCA and VEGF). Following validation at an RNA level we will be in a position to both assess the efficiency with which shRNA clones from the CSHL are working and will be able to use this for small-scale functional analysis.