Performance of Rapid Tests for Detection of Avian Influe

在甲型禽流感病毒亚型中，H5N1和H9N2型病毒有可能导致流感大流行，因为它们在亚洲的禽类中广泛流行，并已显示出感染人类的能力。我们试图确定临床微生物学实验室中常规使用的快速检测方法--使用A549和RhMK细胞的壳瓶检测方法以及两种商用抗原捕获酶免疫分析方法(EIA)是否能够检测出野生型(Wt)和冷适应(Ca)禽流感H5N1和H9N2型病毒。在20,000和20,000 TCID50浓度下，培养1天、2天和5天后，在A549和RhMK细胞中均能很好地检测到H5N1病毒。TCID50为2×10~4的H5N1重组腺病毒在两株细胞中均可检出，但在20×10~4 TCID50时，仅在A549细胞中检测到5天的重组型病毒，在RhMK细胞中的检出时间为2天和5天。在孵育1、2和5天后，两种浓度的H9N2wt病毒在两种细胞系中都能很好地检测到。H9N-ca病毒在20,000 TCID_(50)时也在两种细胞系中检测到，但在20,000 TCID_(50)时，在A549细胞中仅在5天时检测到，在RhMK细胞中在2天和5天时才能检测到。我们发现，对于商业EIA，在50,000 TCID50下，两种检测方法都检测到H9N2wt和ca病毒。在50,000 TCID_(50)时，两种方法均可检测到H5N1 CA病毒，但在该浓度下，两种方法均未检测到wt病毒。

Among the avian influenza A virus subtypes, the H5N1 and H9N2 viruses have the potential to cause an influenza pandemic because they are widely prevalent in avian species in Asia and have demonstrated the ability to infect humans. We sought to determine if rapid assays routinely used in clinical microbiology laboratories, the shell vial assay using A549 and RhMK cells and two commercial antigen capture enzyme immunoassays (EIA), could detect wild type (wt) and cold adapted (ca) avian influenza A H5N1 and H9N2 viruses. At concentrations of 20 and 20,000 TCID50 H5N1 ca virus was detected after 1, 2, and 5 days of incubation equally well in both A549 and RhMK cells. The H5N1 wt virus at 2 x 104 TCID50 was also detected in both cell lines, but at 20 TCID50 the wt virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. The H9N2 wt virus was detected at both concentrations after 1,2, and 5 days of incubation equally well in both cell lines. The H9N ca virus was also detected in both cell lines at 20,000 TCID50, but at 20 TCID50 the ca virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. We found for the commercial EIAs H9N2 wt and ca viruses were detected by both assays at 50,000 TCID50. The H5N1 ca virus was detected at 50,000 TCID50 in both assays, but the wt virus was not detected in either assay at this concentration.