Spectroscopic imaging and diagnosis of neoplasia

描述（由申请人提供）：这是一项向美国国立卫生研究院提出的建立生物工程研究伙伴关系的提案，旨在开发一种用于诊断浸润前肿瘤（发育不良）并监测其进展的光谱成像方法。拟议的计划是基于光学光谱临床仪器和相关的诊断算法成功地开发在麻省理工学院光谱实验室。待开发的仪器将有两个组成部分，一个是基于光散射光谱i（LSS）的瘤形成广域成像系统，一个是基于三模态光谱i（TMS）的用于研究可疑区域的光纤探针设备。该计划的目标是开发和完善新技术，并评估其在真实的时间内对人类患者肿瘤进展的诊断、表征和治疗中的应用。口腔和宫颈肿瘤病变的检测和监测将用作建立该技术潜力的模型系统。此外，还将进行基础研究，以进一步改进该技术及其表征浸润前瘤形成的能力。将开展六个项目，每个项目由一名经验丰富的研究人员领导：（一）将开发、维护和完善用于临床研究的原型仪器和诊断算法。将对（2）口腔和（3）宫颈疑似病变患者进行临床研究，以评估和完善诊断和监测异型增生以及预测患者对化学预防和免疫制剂反应的技术。将开展两个旨在提高临床仪器诊断准确性的基本项目，一个（4）探索使用准多重散射光来提高灵敏度并为LSS成像提供深度分辨率，另一个（5）基于与疾病进展和消退相关的良好表征的分子和细胞事件开发新的光谱终点。(6)病理学支持活动将包括分析口腔和宫颈组织的分子标记物，并通过计算机辅助定量图像分析对同一活检组织的组织切片进行分析。一个行政核心将协调该计划的多学科活动，并确保信息共享和有效沟通。该伙伴关系由六个机构的专家研究人员组成，将包括受过物理和机械/电气工程培训的经验丰富的生物工程师，在癌症研究方面经验丰富的病理学家，以及专门从事口腔和宫颈发育不良的医院临床医生。

DESCRIPTION (provided by applicant): This is a proposal to the National Institutes of Health to establish a Bioengineering Research Partnership to develop a spectroscopic imaging methodology for diagnosing pre-invasive neoplasia (dysplasia) and monitoring its progression. The proposed program is based on optical spectroscopic clinical instrumentation and associated diagnostic algorithms successfully developed at the MIT Spectroscopy Laboratory. The instrument to be developed will have two components, a system for wide-area imaging of neoplasia, based on light scattering spectroscopy i(LSS), and an optical fiber probe device for studying suspect regions thus revealed, based on tri-modal spectroscopy i(TMS). The goal of the program is to develop and perfect the new technology and assess its application to the diagnosis, characterization, and therapy of neoplastic progression in human patients in real time, The detection and monitoring of neoplastic lesions in the oral cavity and the cervix will be used as model systems for establishing the potential of the technology. In addition, basic studies to further improve the technology and its ability to characterize pre-invasive neoplasia will be conducted. Six projects will be undertaken, each led by an experienced investigator: (I) Prototype instruments and diagnostic algorithms for clinical studies will be developed, maintained and perfected. Clinical studies will be conducted on patients with suspected lesions in the (2) oral cavity and (3) uterine cervix to evaluate and perfect the technology for diagnosing and monitoring dysplasia and predicting the patient's response to chemopreventive and immunotherapeutic agents. Two basic projects aimed at enhancing the diagnostic accuracy of the clinical instrumentation will be undertaken, one (4) to explore the use of quasi-multiple scattered light to enhance the sensitivity and provide depth resolution to LSS imaging, and a second (5) to develop novel spectroscopic end-points based on well-characterized molecular and cellular events associated with the progression and regression of disease. (6) Pathology support activities will include analysis of oral and cervical tissues for molecular markers, and analysis of histologic sections of the same biopsy tissue by computer-assisted quantitative image analysis. An administrative core will coordinate the multidisciplinary activities of the program and insure information sharing and efficient communication. The partnership, composed of expert investigators at six institutions, will include experienced bioengineers with training in physics and mechanical/electrical engineering, pathologists experienced in cancer research, and hospital-based clinicians specializing in oral and cervical dysplasia.