Ultrasound monitored HIV rapid test

描述（由申请人提供）：拟议研究的长期目标是开发一种新的定量颗粒凝集技术，以提高体液中人类免疫缺陷病毒（HIV）抗体的检测性能。传统的颗粒凝集检测方法存在灵敏度有限、孵育时间长、主观性强、试剂量大等缺点。正是在这种背景下，我们建议开发一种新技术，它不受上述传统颗粒凝集试验的限制。所提出的免疫测定是一种敏感和快速的方法，使用廉价的仪器。值得注意的是，该方法提供了一种通用的方法来增强和检测不同来源的颗粒的凝集，包括乳胶、二氧化硅微粒和生物细胞。该方法的发展将使人们能够获得定量结果，提高检测抗hiv抗体的颗粒凝集试验的灵敏度，并在不影响每次测试成本的情况下减少分析时间。这些研究的成功完成将提供所需的所有技术，以获得大量商业机会，以许可技术和制造商业产品。

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of detection of human immunodeficiency virus (HIV) antibody in body fluids. The conventional particle agglutination assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propose to develop a new technology, which is free from the aforementioned limitations of the conventional particle agglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. Significantly, the method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silica microparticles, and biological cells. The development of this method will allow one to obtain quantitative results, increase the sensitivity of particle agglutination assays for detection of anti-HIV antibodies, and reduce time of analysis without compromising the cost per test. Successful completion of these studies will make available all of the technology needed for a substantial business opportunity to license the technology and manufacture commercial products.